^(13)N-NH_3心肌灌注显像评价兔心肌梗死模型缺血再灌注  

The experimental study with ^(13)N-NH_3 perfusion imaging to investigate the relationship between ischemic reperfusion and myocardial viability in rabbits with subacute myocardial infarction

作　　者：周谊[1] 乔宏庆[1] 党亚萍 汪静[1] 梁万胜 李国权[1] 卢宁[1] 何蓉[3] 

机构地区：[1]第四军医大学西京医院核医学科,西安710032 [2]长安医院PET中心 [3]第四军医大学西京医院老年病科,西安710032

出　　处：《中华核医学杂志》2004年第3期164-165,i003,共3页Chinese Journal of Nuclear Medicine

摘　　要：目的　探讨兔心肌梗死模型缺血再灌注与梗死面积之间的关系。方法　完全闭塞 16只雄性新西兰大白兔冠状动脉左前降支 4 5min ,随机分为再灌注组 (n =8)和无再灌注组 (n =8) ,7～10d后行1 3N NH3PET CT心肌灌注显像 ,与三氯四氮唑 (TTC)染色梗死灶缺损百分比进行比较。HE染色评价梗死灶病理学改变。结果　TTC染色示无再灌注组、再灌注组实际梗死灶缺损百分比分别为 (2 5 7± 2 3) %、(2 1 5± 2 4 ) % (P =0 0 0 31)。1 3N NH3PET CT心肌灌注显像示 ,无再灌注组与再灌注组缺损百分比分别为 (2 4 6± 2 2 ) %和 (2 0 5± 2 5 ) % (P =0 0 0 37)。组内TTC染色与PET CT显像结果差异无显著性 (P >0 0 5 )。结论　1 3N NH3PET CT心肌灌注显像较TTC染色能更准确测定心肌梗死灶范围。Objective To evaluate the relationship between ischemic reperfusion and infarct size in rabbits with subacute myocardial infarction. Methods 13 N-NH_3 PET/CT imaging was performed on 16 male New Zealand white rabbits of which the left anterior descending coronary artery was completely occluded, non-reperfusion group (n=8), or occluded only for 45 min and after 7～10 d the same operation was repeated, reperfusion group (n=8). The infarct size was determined by staining with triphenyltetrazolium chloride (TTC) or HE staining to identify myocardial changes. Results In non-reperfusion group, the defect percentage was (25.7±2.3)%, but in reperfusion group the defect percent was (21.5±2.4)% (P=0.0031) with TTC staining. Compared the two groups with 13 N-NH_3 imaging the defect area were (24.6±2.2)% and (20.5±2.5)% (P=0.0037). Comparing the TTC staining and PET/CT imaging the defect area percentages were not statistically different within one group. Conclusion Compared with TTC staining, 13 N-NH_3 perfusion imaging measures infarct size more accurately.

关 键 词：^13N-NH3心肌灌注显像 评价 兔 心肌梗死 缺血再灌注 

分 类 号：R817[医药卫生—影像医学与核医学]