咪唑斯汀抑制小鼠变应性接触性皮炎机制的研究  

Experimental Study on Mizolastine inhibiting Allergic Contact Dermatitis in Murine Model

作　　者：陈妍[1] 郭在培[1] 

机构地区：[1]四川大学华西医院皮肤性病科,成都610041

出　　处：《中华皮肤科杂志》2004年第6期344-346,共3页Chinese Journal of Dermatology

摘　　要：目的研究咪唑斯汀对小鼠变应性接触性皮炎(ACD)的抑制作用,探讨其作用机理。方法建立小鼠ACD模型,观察口服不同剂量咪唑斯汀对小鼠耳肿胀的抑制作用。采用ELISA法检测ACD小鼠产生干扰素γ(IFN-γ)、肿瘤坏死因子α(TNF-α)和白介素4(IL-4)的水平及咪唑斯汀对这3种细胞因子的作用。结果口服各剂量咪唑斯汀均能明显抑制ACD小鼠耳肿胀(P<0.05);ACD小鼠血清及脾淋巴细胞IFN-γ水平均明显升高(P<0.01)。ACD小鼠血清TNF-α水平明显升高(P<0.01),腹腔巨噬细胞产生TNF-α水平无明显变化(P>0.05)。ACD小鼠血清及脾淋巴细胞产生IL-4水平无明显变化(P>0.05)。不同剂量咪唑斯汀对3种细胞因子均有显著或不同程度的抑制作用。结论咪唑斯汀对小鼠ACD疗效可能通过其对细胞因子的抑制而发挥作用。Objective To investigate the mechanism of mizolastine inhibiting allergic contact dermatitis (ACD) in murine model. Methods The murine model of allergic contact dermatitis by topical DNFB was used. Using this model murine inhibition of ear swelling was observed after oral administration of mizolastine in different doses. The levels of IFN-γ, TNF-α and IL-4 in the sera of these mice were detected by enzyme-linked immunosorbent assay (ELISA). Results Murine ear swellings were markedly suppressed in each dose group of mizolastine (P < 0.05). In ACD mice the levels of IFN-γ in the serum and the suspensions of spleen cells as well as the levels of TNF-α in serum were significantly increased (P < 0.01), meanwhile the levels of TNF-α from peritoneal macrophages and IL-4 in serum and the suspensions of spleen cells were not changed (P > 0.05). Different doses of mizolastine inhibited the expression of these three cytokines to different degrees. Conclusion The therapeutic effect of mizolastine on murine ACD may be played by inhibiting the expression of some cytokines.

关 键 词：咪唑斯汀 小鼠 变应性接触性皮炎 药物治疗 检测 干扰素Γ 肿瘤坏死因子Α 白介素4 

分 类 号：R758.2[医药卫生—皮肤病学与性病学]