组织工程化骨修复胸壁缺损的实验研究  

Reconstruction of chest wall defect with tissue engineered bone

作　　者：谭强[1] 赵珩[1] 潘银根 施建新[1] 沙慧芳[3] 周允中[1] 

机构地区：[1]上海市胸科医院胸外科,200030 [2]上海市柯隆生物技术公司 [3]上海市胸科医院组织工程实验室,200030

出　　处：《中华胸心血管外科杂志》2004年第3期170-172,共3页Chinese Journal of Thoracic and Cardiovascular Surgery

摘　　要：目的　探索利用组织工程化骨修复胸壁缺损的可行性。方法　实验借助组织工程的基本原理和方法 ,利用猪骨脱细胞基质作为可降解支架材料 ,同时体外扩增自体骨髓间质干细胞作为种子细胞 ,体外构建组织工程化骨组织修复实验用北格犬双侧胸壁各 5cm× 5cm缺损 ,同时在构建的组织工程化骨组织中设计“辅助灌流系统” ,定时注入DMEM培养液、BMP等细胞因子和自体骨髓间质干细胞 ,即保证组织工程化骨中细胞早期营养 ,又维持了局部成骨微环境 ,并同时保证了种子细胞来源。结果　 5只实验动物胸廓未见反常呼吸 ,病理切片证明局部骨组织形成 ;单纯材料对照组可降解支架材料基本吸收 ,伴纤维组织形成。结论　组织工程化骨具有良好的生物相容性 ,并具有一定的强度 ,可防止反常呼吸 。Objective: To discuss the feasibility of using tissue engineered bone to repair large chest wall defects. Methods: Skeletal chest wall defects were reconstructed by tissue engineered bones in five dogs. These tissue engineered bones were constructed by seeding autologous mesenchymal stem cell (MSC) onto xenogenic acellular porcine bone matrix (APBM), a biodegradable scaffold. Within every regenerated bone was and assistant nutrition catheter designed to keep the cell living before reformation of blood supply. This catherter could also maintain the bone regeneration microenvironment through bone morphogenetic protein (BMP) and other growth factors delivered every day. Apparently diminished repair potential may be prevented by periodic administration of MACs. Results: Three-months follow-up showed that sufficient chest wall stability was obtained, and no postoperative complication occurred such as flail chest, infection. Conclusion: Tissue engineered bone is a perfect biomaterial for chest wall defect reconstruction.

关 键 词：组织工程 人工骨 修复 胸壁缺损 实验 胸廓成形术 

分 类 号：R655.1[医药卫生—外科学] R318.1[医药卫生—临床医学]