青春期大鼠实验性精索静脉曲张对附睾细胞线粒体Ca^(2+)和细胞色素C的影响  

Effects of Experimental Varicocele on Mitochondria Calcium and Cytochrome C of the Epididymis in Adolescent Rats

作　　者：马小茹[1] 王淑秋[1] 王跃新[2] 王淑湘[3] 王伟群[1] 张建华[3] 李永义[3] 

机构地区：[1]佳木斯大学基础医学院病理生理学教研室,黑龙江佳木斯154007 [2]佳木斯大学基础医学院生化预防实验室,黑龙江佳木斯154007 [3]佳木斯大学基础医学院机能实验室,黑龙江佳木斯154007

出　　处：《中华男科学杂志》2004年第6期420-422,共3页National Journal of Andrology

基　　金：黑龙江省自然科学基金资助 (D0 0 5 5 )

摘　　要：目的 :探讨外科所致的精索静脉曲张对青春期大鼠附睾细胞线粒体Ca2 + 及细胞色素C的影响。　方法 :对 4 0只青春期雄性Wistar大鼠 ,通过部分结扎左肾静脉或显露左肾静脉建立精索静脉曲张模型 (VG ,2 0只 )和假手术模型 (SOG ,2 0只 ) ,术后 10周 ,取双侧附睾 ,检测附睾头、体部细胞线粒体Ca2 + 、细胞色素C含量。　结果 :与SOG组相比 ,VG组附睾细胞线粒体Ca2 + 含量显著降低 (P <0 .0 0 1) ,细胞色素C含量显著增高 (P <0 .0 5 )。　结论 :外科所致精索静脉曲张大鼠附睾细胞钙稳态失衡、线粒体有损伤 ,这些变化可能是影响其生育能力的机制之一。Objective: To investigate the effects of experimental varicocele on mitochondria calcium and cytochrome C of the epididymal cells in adolescent rats. Methods: Forty male adult Wistar rats were divided into two groups randomly:varicocele group(VG) and sham operation group(SOG) by partial ligation or exposure of the left renal vein. Bilateral epididymides were removed after ten weeks. Mitochondria calcium and cytochrome C levels of the epididymal cells were detected. Results: The content of mitochondria calcium decreased ( P < 0.001 ) while that of cytochrome C increased ( P < 0.05 ) markedly in the experimental group compared with SOG. Conclusion: Calcium dyshomeostasis and mitochondrial damage of the epididymal cells caused by varicocele may play an important role in leading to subfertility.

关 键 词：精索静脉曲张 附睾 线粒体CA^2+ 细胞色素C 细胞凋亡 大鼠 

分 类 号：R697.2[医药卫生—泌尿科学]