树舌多糖对小鼠HepA P16 P27 Rb基因表达的影响  

Influence Of Ganoderm applanatum polysaccharide on tumor suppressor genes P16, P27 and Rb in mice

作　　者：王玉[1] 于英君[2] 徐广有[3] 王璐[4] 周丽[5] 

机构地区：[1]齐齐哈尔医学院生物遗传教研室,黑龙江省齐齐哈尔市161042 [2]黑龙江中医药大学生化教研室,黑龙江省哈尔滨市150040 [3]齐齐哈尔医学院微形态教研室,黑龙江省齐齐哈尔市161042 [4]哈尔滨医科大学国外医学遗传学编辑部,黑龙江省哈尔滨市150086 [5]齐齐哈尔医学院中心实验室,黑龙江省齐齐哈尔市161042

出　　处：《世界华人消化杂志》2004年第6期1353-1356,共4页World Chinese Journal of Digestology

基　　金：黑龙江省教育厅资助课题;No.10511129黑龙江省普通高等学校骨干教师创新能力资助计划;No.1054G070~~

摘　　要：目的:探讨树舌多糖抗肿瘤作用的机制. 方法:通过免疫组化、ELISA法分别测定瘤体和血清中抑癌基因P16,P27,Rb表达蛋白的量;通过透射电镜观察凋亡小体是否存在. 结果:树舌多糖作用后P16,P27,Rb基因表达蛋白的量与盐水组有差异具有统计学意义,免疫组化结果:bP<0.01, vs(1)(2);aP<0.05,vs(2)(3);dP<0.01,vs(3).ELISA结果: aP<0.05,vs(3);bP<0.01,cP<0.05,vs(4);dP<0.01,vs (3)(4);fp<0.01,vs(4);gP<0.05,hP<0.01,vs(3).且P16, P27表达的量与Rb呈负相关mr=-0.094(1),nr=-0.446 (1),再有观察到凋亡小体的存在. 结论:树舌多糖通过激活抑癌基因,抑制肿瘤的生长促进肿瘤细胞凋亡.AIM: To probe into the underlying mechanism of Ganoderm applanatum polysaccharide against cancer. METHODS: By immunohistochemistry and ELISA, we detected the expression of tumor suppressor gene P16, P27 and Rb in tumor tissues and blood serum respectively; By electron microscopy, we observed whether the apoptotic body existed or not in the tumor tissues. RESULTS: After the treatment of Ganoderm applanatum polysaccharide, there was obvious difference in the quantities of P16, P27 and Rb genes expression between the experimental and control groups (P<0.01), and there was a negative correlation of P16 and P27 expression with that of Rb (mr=-0.094,nr=-0.446). We observed the existence of apoptotic body in the tumor cells in the experimental group under electron microscope. CONCLUSION: By activating tumor suppressor gene, Ganoderm applanatum polysaccharide can control the growth of tumor and accelerate the tumor cell apoptosis.

关 键 词：树舌多糖 小鼠 HEPA P16 P27 RB基因表达 免疫组化 细胞周期 

分 类 号：R285.5[医药卫生—中药学]