复方红景天对大鼠高原严重烧伤后早期心肌损伤的保护作用  

The protective effect of Rhadiola Astragalus Codonopsis Compound on myocardium in early stage of severe burn at plateau in rats

作　　者：于晟[1] 薛晓东[2] 杨生兰 张世范[1] 刘毅[1] 朱云[1] 蔡黔[1] 张绪生[1] 张鲜英[1] 钟晓玲[1] 

机构地区：[1]兰州军区总医院烧伤科,兰州730050 [2]甘肃省人民医院烧伤科 [3]甘肃省建筑职工医院病理科

出　　处：《中华烧伤杂志》2004年第3期134-137,共4页Chinese Journal of Burns

基　　金：全军"十五"指令性课题资助项目 (0 1L0 0 3)

摘　　要：目的　观察高海拔 (3480m)地区大鼠 30 %TBSAⅢ度烧伤后心肌损害的程度 ,探讨使用复方红景天后对大鼠心肌损伤的作用机制。　方法　 10 4只Wistar大鼠 ,随机分为红景天组 (48只 )、盐水组 (48只 )、正常对照组 (8只 )。正常对照组大鼠不作烧伤和其他处理。红景天组伤前 1周向大鼠胃内灌注复方红景天液 4ml,2次 /d;盐水组同方法灌注等量等渗盐水。分别在伤后 3、6、12、2 4、4 8、72h(每时相点 8只大鼠 )剖腹抽血 ,检测大鼠心肌酶谱及动脉血气变化 ,再摘取大鼠心脏作病理学检查。　结果　烧伤后 3h盐水组大鼠心肌组织损害明显 ,随后逐渐减轻 ,伤后 72h接近正常对照组水平。红景天组与盐水组大鼠心肌酶谱值均明显高于正常对照组 (P <0.0 1),但红景天组则明显低于盐水组 (P <0.0 1)。血气分析 :pH值 :盐水组与红景天组均低于正常对照组 (P <0.0 5 ),红景天组伤后 12～ 2 4h高于盐水组 (P <0.0 5)。剩余碱 :盐水组与红景天组均明显低于正常对照组 (P <0.0 1)。红景天组伤后 6h后高于盐水组 (P <0.0 5～ 0.0 1)。二氧化碳分压 :盐水组与红景天组均明显低于正常对照组 (P <0.0 5～ 0.0 1),伤后 4 8h红景天组 (35 .70± 4 .2 3)mmHg(1mmHg =0.133kPa)与正常对照组 (37.5 0± 6 .5 3)mmHg比较 。Objective To investigate the severity of early myocardial injury in rats with 30% full thickness burn at plateau and the protective effects of Rhadiola Astragalus Codonopsis Compound (RACC) on the rat myocardial injury. Methods One hundred and four Wistar rats with 30% full thickness burn were randomly divided into RACC application (R, n=48) and scalding group 1 (S, n=48), and another 8 healthy Wistar rats as control group 2 (C, n=8). Four ml of RACC was garaged into the rat stomach in R and 4 ml isotonic saline in S groups respectively, but no treatment in C group. Blood samples from the aorta were harvested in 3, 6, 12, 24, 48 and 72 postburn hours (PBH) for blood gas analysis and for the determination of the changes in myocardial enzymes. Rat heart was harvested for pathomorphological examination. Results The rat myocardial tissue injury in R and S groups was obvious at 3 PBH and ameliorated gradually thereafter, up to the degree in C group at 72 PBH. The serum levels of myocardial enzymes in R and S groups were significantly higher than those in C group ( P < 0.01). Whereas the enzymes in R group were much lower than those in S group ( P < 0.01). It was indicated by blood gas analysis that the pH in R and S groups was lower than that in C group( P < 0.05), while that in R group at 12-24 PBH was higher than that in S group ( P < 0.05) . In addition, the base excess in R and S groups was lower than that in C group ( P < 0.01), while that in R group at 6 PBH was higher than that in S group ( P < 0.05～0.01). The PaCO 2 in R and S groups was evidently lower than that in C group ( P < 0.05～0.01), while that in R group at 48 PBH was no different to that in C group( 35.70±4.23 mmHg vs 37.50±6.53 mmHg, P > 0.05 ). The PaO 2 in R and S groups at 3～24 PBH was higher than that in C group and decreased gradually( P >0.05). There was no difference in SaO 2 among 3 groups( P >0.05). Conclusion RACC exhibited beneficial to the protection of rat heart from myocardial injury at plateau induced by severe burn.

关 键 词：复方红景天 大鼠 高原 烧伤 心肌损伤 保护作用 

分 类 号：R644[医药卫生—外科学]