高效液相色谱法测定参芨口服液中原儿茶醛的含量  

Determination of Protocatechuic Aldehyde in Shenji Oral Liquid by HPLC

作　　者：史成和[1] 李进华[1] 于晓峰[2] 陆松敏[1] 

机构地区：[1]第三军医大学大坪医院野战外科研究二室,重庆400042 [2]浙江省儿童医院药剂科,浙江杭州310000

出　　处：《中国药业》2004年第7期33-34,共2页China Pharmaceuticals

摘　　要：目的测定参芨口服液中原儿茶醛含量。方法采用高效液相色谱法,以甲醇-水-冰醋酸(25∶75∶0.2)为流动相,流速为1mL/min,检测波长为281nm。结果原儿茶醛的线性范围为0.45～2.7μg/mL,平均回收率为98.9%,RSD=1.87%,r=0.9996。结论高效液相色谱法测定原儿茶醛含量,方法快速,结果准确,操作简便,可用于产品质量研究。Objective:To determine the content of protocatechuic aldehyde in Shenji oral liquid.Methods:A HPLC method was used.The mobile phase consisted of methgl alcohol-water-acetic acid(25 ∶75 ∶0.2).The flow rate was 1 mL/min.The detection wavelength was at 281 nm.Results:The linear range of protocatechuic aldehyde was 0.45～2.7 μg/mL.The average recovery rate was 98.9%(RSD=1.87%). Conclusion:The HPLC method is accurate,simple and suitable for quality studies of the oral liquid.

关 键 词：参芨口服液 原儿茶醛 含量测定 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]