107杨次生木质部PAL基因的RT-PCR扩增及其鉴定  

Cloning and Identification of PAL Gene Amplified by RT-PCR from Populus×euramericana cv.“74/76” Second Xylem mRNA

作　　者：薛永常[1] 李金花[2] 卢孟柱[2] 张绮纹[2] 

机构地区：[1]大连轻工业学院生物与食品学院,大连116034 [2]中国林业科学研究院林业研究所,北京100091

出　　处：《林业科学》2004年第4期193-197,共5页Scientia Silvae Sinicae

基　　金：国家林业局 948项目---控制林木木质素合成酶基因的引进及其转化技术研究 ( 94-4 -2 1)

摘　　要：A fragment of PAL (phenylalanine ammonia_lyase) gene was amplified by RT_PCR from poplar (Populus×euramericana cv. “74/76”) developing second xylem mRNA. It was cloned into pGEM-T Easy vector and identified by restriction enzyme, PCR amplification and sequencing. The sequence of the amplified DNA fragment was 565 base pairs. Alignment with the P. kitakamiensis PAL cDNA sequence retrieved from EMBL nucleotide acid database (accession number D30656) showed that the first 400 base pairs in both sequences were almost identical. Therefore the fragment was part of PAL gene. And both of sense and anti-sense expressional vectors were constructed.A fragment of PAL (phenylalanine ammonia_lyase) gene was amplified by RT_PCR from poplar (Populus×euramericana cv. “74/76”) developing second xylem mRNA. It was cloned into pGEM-T Easy vector and identified by restriction enzyme, PCR amplification and sequencing. The sequence of the amplified DNA fragment was 565 base pairs. Alignment with the P. kitakamiensis PAL cDNA sequence retrieved from EMBL nucleotide acid database (accession number D30656) showed that the first 400 base pairs in both sequences were almost identical. Therefore the fragment was part of PAL gene. And both of sense and anti-sense expressional vectors were constructed.

关 键 词：次生木质部 PAL基因 RT-PCR 鉴定 苯丙氨酸解氨酶 欧美杨107 

分 类 号：S792.11[农业科学—林木遗传育种]