天麻抗真菌蛋白基因(GAFP)转化彩色棉新品系抗黄、枯萎病性鉴定  

The Resistance Identification to Verticillium wilt and to Fusarium wilt for Transgenic GAFP Colored Cotton Lines of Xinjiang

作　　者：刘丰疆 马德成 李仁敬 李卫平[3] 陈大军 姚正培 宋武 李玮 

机构地区：[1]新疆中国彩棉集团股份有限公司,新疆乌鲁木齐830016 [2]新疆植物保护站,新疆乌鲁木齐830006 [3]巴音郭楞州农科所,新疆库尔勒841000

出　　处：《新疆农业科学》2004年第4期244-247,共4页Xinjiang Agricultural Sciences

基　　金：国家技术创新项目(00C26216501357)

摘　　要：将天麻抗真菌蛋白基因(GastrodiaAntifargalProtein,GAFP)利用花粉管通道法导入彩色棉品种新彩棉1号和新彩棉3号,转化后代经卡那霉素初筛、分子检测、农艺性状选择,培育出16个转基因新品系,对其在人工病圃进行田间黄、枯萎病的抗性鉴定,结果表明:参试的转基因品系中LB5007、ZB5020等5个品系既抗黄萎病又抗枯萎病,为兼抗类型;其余11个品系均为抗黄萎病耐枯萎病类型。The gene of Gastrodia Antifargal Protein (GAFP) was introduced into colored cotton species of No.1 New colored cotton (brown) and No.3 New colored cotton (green) in Xinjiang by using pollen-tube pathway method. Through kanamycin check, PCR-southern blot test and agronomic character identification, 16 new lines of colored cotton which have been integrated GAFP gene were obtained. The resistance identification to Verticillium wilt and to Fusarium wilt in the diseased field nursery proved that: the lines of LB5007, ZB5020 were resistant to both Fusarium wilt and to Verticillium wilt, the others lines were resistant to Fusarium wilt and tolerant to Verticillium wilt.

关 键 词：天麻抗真菌蛋白基因 彩色棉 黄萎病 枯萎病 抗性鉴定 转基因 

分 类 号：S435.621.2[农业科学—农业昆虫与害虫防治] S562[农业科学—植物保护]