Comparison of the Contributions of Tetrahydrofurfuryl Alcohol and PEG to a-Chymotrypsin Renaturation with High Performance Hydrophobic Interaction Chromatography  

作　　者：Ye Hua SHEN Hai Bo WANG Quan BAI Xin Du GENG 

机构地区：[1]中国人民解放军85医院，上海200052

出　　处：《Chinese Chemical Letters》2003年第3期294-297,共4页中国化学快报（英文版）

基　　金：These projects No. 39880003 and 20175016 were supported by the National Natural Science Foundation of China.

摘　　要：The contributions of tetrahydrofurfuryl alcohol (THFA) and polyethylene glycol (PEG) to the renatured efficiency of a-chymotrypsin were investigated and compared with each other. The maximum increments of bioactivity recovery of a-Chy were found to be 25.1% for THFA, 10.4% for PEG, respectively. The experimental results indicated that the denaturant solution containing THFA contributed more to the renaturation of a-Chy in high performance hydrophobic interaction chromatography (HPHIC) than that containing PEG, when the concentration of THFA was 3.2%, the bioactivity recovery of a-Chy is the highest.The contributions of tetrahydrofurfuryl alcohol (THFA) and polyethylene glycol (PEG) to the renatured efficiency of a-chymotrypsin were investigated and compared with each other. The maximum increments of bioactivity recovery of a-Chy were found to be 25.1% for THFA, 10.4% for PEG, respectively. The experimental results indicated that the denaturant solution containing THFA contributed more to the renaturation of a-Chy in high performance hydrophobic interaction chromatography (HPHIC) than that containing PEG, when the concentration of THFA was 3.2%, the bioactivity recovery of a-Chy is the highest.

关 键 词：High-perforamnce hydrophobic interaction chromatography tetrahydrofurfuryl alcohol (THFA) polyethylene glycol (PEG) protein renaturation a-chymotrypsin. 

分 类 号：Q51[生物学—生物化学]