Study on the Enhancement of Proton Affinity by N-Diisopropyloxy Phosphorylation of Amino Acid in Mass Spectrometry  

作　　者：Hai LIU Jing CHEN 等 

机构地区：[1]TheKeyLaboratoryofBioorganicPhosphorusChemistry,MinistryofEducation,DepartmentofChemistry,SchoolofLifeSciencesandEngineering,TsinghuaUniversity,Beijing100084 [2]TheKeyLaboratoryofBi

出　　处：《Chinese Chemical Letters》2003年第2期195-196,共2页中国化学快报（英文版）

基　　金：the National Natural Science Foundation of China (No. 20175026); the Ministry of Science and Technology of China; the Education Ministry of China and Tsinghua University for financial support.

摘　　要：With introduction of a diisopropyloxy phosphoryl group into the N terminal of amino acids, it was found that proton affinity (PA) of amino acid was enhanced in mass spectrometry. Density functional theory calculations showed that the energy for protonation of DIPP-amino acid is lower than that of amino acid, which means PA of DIPP-AA is higher than that of corresponding amino acid. These results, coincident with our empirical results, offer a useful interpretation of experimental observations.With introduction of a diisopropyloxy phosphoryl group into the N terminal of amino acids, it was found that proton affinity (PA) of amino acid was enhanced in mass spectrometry. Density functional theory calculations showed that the energy for protonation of DIPP-amino acid is lower than that of amino acid, which means PA of DIPP-AA is higher than that of corresponding amino acid. These results, coincident with our empirical results, offer a useful interpretation of experimental observations.

关 键 词：Proton affinity diisopropyloxy phosphoryl group amino acid mass spectrometry. 

分 类 号：Q517[生物学—生物化学] Q503