Comparison of Frontal Analysis and Elution-Curve Method for Determining Adsorption Isotherms  

作　　者：Ju-Weon LEE Kyung Ho ROW 

机构地区：[1]Center for Advanced Bioseparation Technology Department of Chemical Engineering,Inha University,Center for Advanced Bioseparation Technology Department of Chemical Engineering,Inha University Incheon 402-751,Korea,Incheon 402-751,Korea

出　　处：《Chinese Journal of Chemical Engineering》2002年第6期720-722,共3页中国化学工程学报（英文版）

摘　　要：Adsorption isotherm is the most fundamental information related to chromatography. To calculate the parameters of Langmuir ad-sorption isotherm of thymidine, frontal analysis (FA) and elution-curve method (ECM) were adopted in reversed-phase high performance liguid chromatography (RP-HPLC). In FA, the concentration of stationary phase was measured from the elution curves and the isotherm was deter-mined by regression analysis, while the parameters by ECM were obtained by parameter optimization. The adsorption isotherms of thymidine from the two methods were very similar. The superiority of ECM over FA was that the consumption of sample was less and only one or two in-jections of sample were required.Adsorption isotherm is the most fundamental information related to chromatography. To calculate the parameters of Langmuir ad-sorption isotherm of thymidine, frontal analysis (FA) and elution-curve method (ECM) were adopted in reversed-phase high performance liguid chromatography (RP-HPLC). In FA, the concentration of stationary phase was measured from the elution curves and the isotherm was deter-mined by regression analysis, while the parameters by ECM were obtained by parameter optimization. The adsorption isotherms of thymidine from the two methods were very similar. The superiority of ECM over FA was that the consumption of sample was less and only one or two in-jections of sample were required.

关 键 词：frontal analysis elution-curve method adsorption isotherm reversed-phase high performance liguid chromatography * 

分 类 号：TQ02[化学工程]