Regeneration of fertile plants from isolated tobacco zygotes by in vitro culture  

作　　者：HEYuchi SUNMengxiang YANGHongyuan 

机构地区：[1]KeyLaboratoryofMOEforPlantDevelopmentalBiology,WuhanUniversity,Wuhan430072,China

出　　处：《Chinese Science Bulletin》2004年第8期810-814,共5页

摘　　要：Living zygotes of tobacco (Nicotiana Tabacum L.) SR-1 were isolated and cultured in vitro by the microcul-ture technique. Fertile plants were regenerated from the calli derived from cultured zygotes via organogenesis. Ovules were collected 120 h after pollination and used as feeder. MS combined with KM8p was selected as basic medium in the experiment. Zygotes isolated from ovules 108 h after pollina-tion turned out to be suitable material for in vitro culture. Over 80% such zygotes could divide and around 10% of them could grow into calli and regenerate fertile plants.Living zygotes of tobacco (Nicotiana Tabacum L.) SR-1 were isolated and cultured in vitro by the microcul-ture technique. Fertile plants were regenerated from the calli derived from cultured zygotes via organogenesis. Ovules were collected 120 h after pollination and used as feeder. MS combined with KM8p was selected as basic medium in the experiment. Zygotes isolated from ovules 108 h after pollina-tion turned out to be suitable material for in vitro culture. Over 80% such zygotes could divide and around 10% of them could grow into calli and regenerate fertile plants.

关 键 词：烟草 接合子 微晶结构 植物再生 孢子体 基因转换 

分 类 号：S572[农业科学—烟草工业] Q944.4[农业科学—作物学]