不同来源树突状细胞的培养研究  

Research on culturing dendritic cells from different sources

作　　者：翁霞[1] 邓晓辉[1] 马静秋[1] 余金妹[1] 梁辉[1] 

机构地区：[1]上海第二医科大学新华医院血液科,上海200092

出　　处：《上海第二医科大学学报》2004年第5期331-333,共3页Acta Universitatis Medicinalis Secondae Shanghai

基　　金：上海市科技发展基金资助项目(4119024)

摘　　要：目的 比较不同来源的树突状细胞(DC)在体外扩增、分化成熟的特点。方法 将骨髓和外周血采集物作为DC的培养源,联合GM-CSF、IL-4细胞因子培养DC,观察培养过程中DC的超微结构变化,检测其免疫表型。结果 外周血采集物培养的DC成熟明显早于骨髓采集物培养的DC(P<0.01)。DC表型CD1a-PE、HLA-DR-PE、CD54-FITC、CD80-FITC的阳性率平均值在骨髓组和外周血组分别为23.3±3.6和25.8±2.2、95.0±2.1和96.9±1.0、54.4±1.4和56.1±1.2、40.5±2.7和41.7±1.8(P>0.05)。结论 外周血组DC表型的阳性率稍高于骨髓组,但获取费用较昂贵,适用范围有限;骨髓来源则较简便,但培养成熟时间较长,且受个体差异的限制。Objective To compare the growing behavior of dendritic cell (DC) from different sources. Methods DCs were cultured from bone marrow and leukapheresis products with the existence of GM-CSF and IL-4. The morphology and ultrastructure were studied and the immune phenotypes of DC were analysed. Results Maturity of DC in the leukapheresis products was much quicker than in bone marrow (P < 0. 01). The positive rate of CDla-PE, HLA-DR-PE,CD54-FITC,CD80-FITC of DC between bone marrow and leukapheresis products was 23.3±3.6 vs 25.8±2.2, 95.0±2.1 vs 96. 9±1.0, 54.4±1.4 vs 56. 1±1.2, 40.5±2.7 vs 41.7±1.8, respectively (P> 0.05). Conclusion Maturity of DC in leukapheresis products is much quicker than in bone marrow, but leukapheresis is expensive. The source of bone marrow is more convenient but the maturity time of DC is longer.

关 键 词：树突状细胞 研究方法 材料来源 细胞培养 DC表型检测 骨髓 外周血 

分 类 号：Q813.11[生物学—生物工程]