Regulation of BMP4 on the proliferation and differentiation in SVZa neural stem cells  

作　　者：LIUShiyong ZHANGZhiyuan SONGYechun QIUKejun ZHANGKecheng ANNing ZHOUZheng CAIWenqin YANGHui 

机构地区：[1]DepartmentofNeurosurgery,XinQiaoHospital,theThirdMilitaryMedicalUniversity,Chongqing400037,China [2]LaboratoryofNeurobiology,theThirdMilitaryMedicalUniversity,Chongqing400038,China

出　　处：《Chinese Science Bulletin》2004年第11期1126-1136,共11页

摘　　要：The neural stem cells in the anterior subventricula zone (SVZa) mainly generate the progenitors that will differentiate into neurons, and along a highly circumscribed migratory access-Rostral migratory stream (RMS), they migrate to the olfactory bulbs (OB). To understand the effects of BMPs on SVZa neural stem cells, in this study BMP4 at various concentrations was used to induce SVZa ,neural stem cells, aud the living cell labeling using BMP4 pronmtor conjugated with red fluorescence protein showed the expression of BMP4 dynamically. The results demonstrated that low BMP4 doses (1-5 ng/mL) promoted while high doses(10-100 ng/mL) inhibited the proliferation of SVZa neural stem cells, and BMP4 promoted neuron differentiation in the early stage (1-3 d), howeverm, it inhibited the neuron commitment after 4 d. Noggin, the antagonist of BMP4, blocked the physiological effects of BMP4. In OB, BMP4 is mainly to accelerate the progenitors to withdraw from the cell cycle and trigger the differentiation, and in RMS, it promotes the proliferation of committed progenitors and not differentiation, further in SVZa, BMP4 enhances astrocyte commitment.The neural stem cells in the anterior subventricula zone (SVZa) mainly generate the progenitors that will differentiate into neurons, and along a highly circumscribed migratory access-Rostral migratory stream (RMS), they migrate to the olfactory bulbs (OB). To understand the effects of BMPs on SVZa neural stem cells, in this study BMP4 at various concentrations was used to induce SVZa ,neural stem cells, aud the living cell labeling using BMP4 pronmtor conjugated with red fluorescence protein showed the expression of BMP4 dynamically. The results demonstrated that low BMP4 doses (1-5 ng/mL) promoted while high doses(10-100 ng/mL) inhibited the proliferation of SVZa neural stem cells, and BMP4 promoted neuron differentiation in the early stage (1-3 d), howeverm, it inhibited the neuron commitment after 4 d. Noggin, the antagonist of BMP4, blocked the physiological effects of BMP4. In OB, BMP4 is mainly to accelerate the progenitors to withdraw from the cell cycle and trigger the differentiation, and in RMS, it promotes the proliferation of committed progenitors and not differentiation, further in SVZa, BMP4 enhances astrocyte commitment.

关 键 词：神经干细胞 分芽繁殖 骨形态发生蛋白 红荧光蛋白 绿荧光蛋白 前向次心室带 

分 类 号：Q421[生物学—神经生物学] R322.8[生物学—生理学]