毒物代谢酶基因多态性与职业性慢性苯中毒危险性的单纯病例研究  

Case-only study on the relationship between genetic polymorphisms in toxicant metabolizing enzymes and risk of occupational chronic benzene poisoning

作　　者：张忠彬[1] 万俊香[1] 夏昭林[1] 

机构地区：[1]复旦大学公共卫生学院劳动卫生教研室,上海200032

出　　处：《中华劳动卫生职业病杂志》2004年第3期168-172,共5页Chinese Journal of Industrial Hygiene and Occupational Diseases

基　　金：国家自然科学基金资助项目 ( 3 0 2 71113 )

摘　　要：目的　研究环境暴露因素与毒物代谢酶基因多态性对发生慢性苯中毒危险性的影响。方法　以 15 2例慢性苯中毒工人为研究对象 ,应用单纯病例方法进行分析。结果　中等强度接触组慢性苯中毒工人GSTT1基因缺失突变的发生率明显高于低接触组 ,差异有显著性 (6 8.6 3%vs38.0 0 % ,ORadj=4 .32 ,95 %CI 1.75～ 10 .6 6 ,P =0 .0 0 2 ) ;NQO1C .6 0 9T T基因在饮酒组的比例明显高于不饮酒组 ,差异有显著性 (6 1.11%vs 2 0 .0 0 % ,ORadj=8.0 3,95 %CI 2 .2 8～ 2 8.2 5 ,P =0 .0 0 1) ,在吸烟又同时饮酒组的比例明显高于其他组 ,差异有显著性 (85 .71%vs 2 2 .76 % ,ORadj=18.6 2 ,95 %CI 2 .0 1～172 .72 ,P =0 .0 1) ,在饮酒×接触等级组的比例明显高于非饮酒×接触等级组 ,差异有显著性(6 1.11%vs 2 0 .0 0 % ,ORadj=3.18,95 %CI 1.5 5～ 6 .5 2 ,P =0 .0 0 2 ) ;GSTM1基因缺失突变在饮酒×接触等级组的比例明显高于非饮酒×接触等级组 ,差异有显著性 (6 6 .6 7%vs 4 7.0 6 % ,ORadj=1.99,95 %CI 1.0 5～ 3.76 ,P =0 .0 36 )。结论　GSTT1的基因多态与中等强度苯接触存在交互作用 ;不同强度的苯接触同饮酒一起与GSTM1基因缺失突变增加了个体发生慢性苯中毒的风险性 ;NQO1基因C .6 0 9位点的多态与饮酒存在交?Objective To explore the effects of interaction between environmental exposure factors and genetic polymorphism in toxicant metabolizing enzymes on risk of occupational chronic benzene poisoning. Methods One hundred and fifty-two cases of chronic benzene poisoning were analyzed for the risk by case-only study. Results The frequency of non-null GSTT1 gene in benzene poisoning workers with moderate benzene exposure level was higher than that in cases with lower benzene exposure(68.63% vs 38.00%,OR adj =4.32,95%CI 1.75～10.66,P=0.002).The frequency of NQO1 C.609T/T gene in alcohol drinking group was higher than that in non-drinking group(61.11% vs 20.00%,OR adj =8.03,95%CI 2.28～28.25,P=0.001),moreover,it was higher in workers with smoking and drinking than that in the rest group,and in drinking×exposure level workers than that in non-drinking×exposure level workers(85.71% vs 22.76%,OR adj =18.62,95%CI 2.01～172.72,P=0.01 and 61.11% vs 20.00%,OR adj =3.18,95%CI 1.55～6.52,P=0.002 respectively).The frequency of non-null GSTM1 gene was also higher in drinking×exposure level workers than that in non-drinking×exposure level workers(66.67% vs 47.06%,OR adj =1.99,95%CI 1.05～3.76,P=0.036). Conclusion There is interaction between the polymorphism of GSTT1 gene and moderate benzene exposure level;non-null GSTM1 gene and drinking×exposure level increase the risk of occupational chronic benzene poisoning;polymorphism of NQO1 gene C.609 also interacts with drinking,while polymorphism of NQO1 gene and drinking×smoking may further increase the risk of occupational chronic benzene poisoning.

关 键 词：毒物代谢酶 基因多态性 职业性慢性苯中毒 吸烟 饮酒 苯接触 交互作用 

分 类 号：R135[医药卫生—劳动卫生]