腺病毒介导CD40Ig基因转染联合骨髓细胞输注诱导大鼠心脏移植物耐受的研究  

Effects of adenovirus mediated CD40Ig gene transfer combining with bone marrowtransfusion on induction of rat heart allograft tolerance

作　　者：徐衍盛[1] 肖序仁[1] 李峰永[2] 

机构地区：[1]北京市解放军总医院泌尿外科,北京市100853 [2]吉林大学第三临床医院

出　　处：《中国实验诊断学》2004年第3期267-269,共3页Chinese Journal of Laboratory Diagnosis

摘　　要：目的　研究CD4 0Ig基因转染联合骨髓细胞输注对大鼠心脏移植免疫耐受诱导的影响。方法　构建可表达CD4 0Ig的腺病毒载体Ad CD4 0Ig。建立大鼠心脏移植模型 ,将实验动物分为 5组 :A ,对照组 ,无任何处理 ;B组 ,单纯输注供者骨髓细胞 ;C ,单纯输注Ad CD4 0Ig ;D ,Ad CD4 0Ig和供者的骨髓细胞联合输注 ;E :输注对照腺病毒Ad Lacz。观察心脏移植物的存活时间、并MLR检测及移植物组织学检查。结果　 5组移植物的存活时间分别为 :8 34± 0 35天、9 2 6± 0 84天、18 73± 1 5 7天、36 91± 4 2 0天和 7 87± 0 4 1天。D组移植物的存活时间显著延长 ,MLR表现出供者特异的低反应性 ,组织切片检查淋巴细胞浸润情况也明显减轻。结论　腺病毒介导CD4 0Ig基因转染联合骨髓细胞输注 ,可诱导一定程度的免疫耐受。Objective To investigate the effects of adenovirus mediated CD40Ig gene transfer combining with bone marrow transfusion on induction of rat heart allograft tolerance.Methods We constructed a recombinant adenovirus vector Ad/CD40Ig that can express CD40Ig and set up cardiac transplant model. The experimental rats were divided into five groups. A:without any treatment; B:intravenous injection of donor bone marrow cells 1×10 7; C:intravenous injection of Ad/CD40Ig 2×10 8 FU; D:intravenous injection of Ad/CD40Ig 2×10 8 FU and donor bone marrow cells 1×10 7;Group D:Intravenous injection of Ad/Lacz 2 ×10 8 FU. The survival days of allograft、MLR and histological changes were observed.Results The survival days in group A、B、C、D、E were(8.34±0.35d)、(9.26±0.84d)、(18.73±1.57d)、(36.91±4.20d) and (7.87±0.41d) respectively. The survival time in group D was obviously prolonged than that in other four groups. MLR appeared donor special low-response, and lymphocytes infiltration was ameliorated in histological test.Conclusion Conclusion Donor special bone marrow infusion can synergistically enhance the power of CD40Ig gene transfer to induce heart allograft tolerance.

关 键 词：腺病毒载体 基因转染 骨髓输注 免疫耐受 

分 类 号：R654.2[医药卫生—外科学]