大鼠心内神经节雄激素受体和神经生长因子的共存  

Coexistence of androgen receptor and nerve growth factor in intrinsic cardiac ganglia of rats

作　　者：刘晓柳[1] 杨洁[2] 李光千[3] 赵小明[3] 

机构地区：[1]武汉科技大学医学院解剖学教研室,武汉4300803 [2]湖北省十堰市人民医院呼吸内科 [3]华中科技大学同济医学院解剖学系

出　　处：《解剖学杂志》2004年第3期277-279,316,共4页Chinese Journal of Anatomy

摘　　要：目的 :研究雄激素受体 (AR)和神经生长因子 (NGF)的表达 ,证实它们在心内神经节共存的可能性。方法 :免疫细胞化学双重标记技术。结果 :切片上观察到 3种细胞 :( 1 )AR单标细胞 ,胞核呈棕褐色 ;( 2 )NGF单标细胞 ,胞浆呈红色 ;( 3 )AR/NGF双标细胞 ,胞核棕褐色 ,胞浆为红色。双标细胞占全部阳性标记细胞的 5 0 %～ 60 %。结论 :AR和NGF可共存于同一大鼠心内神经节细胞 。Objective: To investigate the expression of androgen receptor (AR)and nerve growth factor(NGF)in the intrinsic cardiac ganglia of rats and to identify the possibility of coexistence of them in the intrinsic cardiac ganglia. Methods: The double labeling immunocytochemical staining technique was used. Results: There were 3 immunoreactive(ir) labeled neurons in the intrinsic cardiac ganglia.The AR ir production showed brown in color,located in the nucleus;the NGF ir production showed red in color,located in the cytoplasm;there were AR/NGF double labeled cells in same section,which accounted for about 50% 60% of the total single and double labeled neurons. Conclusion: AR and NGF can coexist in same rat intrinsic cardiac ganglia,suggesting that there may be complex interactions between androgen and neurotrophic factors in maintaining intrinsic cardiac ganglia neuronal structure and function.

关 键 词：大鼠 心内神经节 雄激素受体 神经生长因子 NGF AR 免疫组织化学 心脏神经结构 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]