3种生物制剂对芥子气眼角膜损伤的疗效观察  

THERAPEUTIC EFFECTS OF THREE BIOLOGICAL PREPARATIONS ON MUSTARD GAS INDUCED CORNEAL WOUND IN RABBIT

作　　者：蔡季平[1] 赵杰[2] 胡维国[2] 朱明学[2] 

机构地区：[1]第二军医大学附属长征医院眼科,上海200003 [2]第二军医大学海医系防化医学教研室

出　　处：《解放军预防医学杂志》2004年第3期178-180,共3页Journal of Preventive Medicine of Chinese People's Liberation Army

基　　金：全军"十五"指令性课题 (No.0 1L0 5 4)

摘　　要：目的观察碱性成纤维细胞生长因子 (bFGF)、表皮生长因子 (EGF)和纤维连接蛋白(FN) 3种促进上皮再生的生物制剂对兔眼角膜芥子气损伤的疗效。方法将 32只兔左眼角膜以芥子气染毒后 ,随机均分为 4组 ,分别用氯霉素眼液 (对照 )、bFGF、EGF及FN处理 ,分别于染毒后 2、8、16、2 4、36、4 8和 72h对角膜荧光素着色区拍照 ,计算其上皮愈合速率和上皮破损率。结果bFGF、EGF和FN治疗组的角膜上皮愈合速率 (3d)分别为 (1.2 76± 0 .15 2 )、(1.2 94± 0 .15 8)和(1.373± 0 .177)mm2 ·h 1,与对照组的 (1.0 94± 0 .15 4)mm2 ·h 1相比有显著差异 (P <0 .0 5 ) ;3个组的角膜上皮破损率 (2 7d)分别为 5 1.9%、5 4.2 %和 5 2 .8% ,与对照组的 (73.6 % )相比有非常显著差异 (P <0 .0 1)。结论bFGF、EGF和FNObjective To observe the therapeutic effects of basic fibroblast growth factor (bFGF), epidermal growth factor (EGF),and fibronectin (FN) on rabbit corneal wound caused by mustard gas. Methods After the left eye cornea were exposed to mustard gas (200 μL·L 1 ), 32 rabbits were divided into four groups (group A, B, C, D) at random. Group A, B, C, D were treated with chloromycetin eyedrops (control), bFGF, EGF and FN respectively. The cornea were stained with fluorescence and photographed 2, 8, 16, 24, 36,72 h after exposure. The rate of corneal epithelium healing and percentage of corneal defect were calculated. Results The epithelium healing rates of treated groups(B, C, D) for three days 〔(1.276±0.152)mm 2·h 1 ,(1.294±0.158)mm 2·h 1 , and (1.373±0.177)mm 2·h 1 respectively〕were higher than that of control〔(1.094±0.154)mm 2·h 1 ,P<0.05〕. The percentages of corneal defect of treated groups for 27 days(51.9%、54.2% and 52.8%) were lower than that of control (73.6%, P<0.01). Conclusion The treatment with bFGF、EGF and FN could accelerate the healing of corneal epithelium injured by mustard gas.

关 键 词：成纤维细胞生长因子(bFGF) 表皮生长因子(EGF) 纤维连接蛋白(FN) 芥子气 

分 类 号：R827.17[医药卫生—临床医学]