碱性成纤维细胞生长因子对体外培养成年大鼠海马神经前体细胞ERK1/2活性的影响  

Effect of Basic Fibroblast Growth Factor on the Activity of ERK1/2 in Cultured adult Hippocampus Neural Precursor Cells

作　　者：程祖珏[1] 王琳[2] 王卫东[1] 徐江平[3] 邵卫中[1] 余永琴[1] 

机构地区：[1]武警江西总队医院急诊科,江西南昌330001 [2]江西医学院第二附属医院急诊科,江西南昌330006 [3]第一军医大学药理学教研室,广东广州510515

出　　处：《江西医学院学报》2004年第3期33-36,共4页Acta Academiae Medicinae Jiangxi

摘　　要：目的　观察碱性成纤维细胞生长因子 (bFGF)对体外培养成年大鼠海马神经前体细胞增殖和细胞外信号调节蛋白激酶 (ERK)活性的影响。方法　体外培养成年大鼠海马神经前体细胞经预处理后 ,分别加入BrdU(10μmol)和bFGF(2 0ng/ml)进行培养 ,采用流式细胞仪和Westernblot方法测定海马神经前体细胞DNA合成和磷酸化ERK1/ 2活性。结果　流式细胞仪结果显示加入bFGF进行培养的海马成年神经前体细胞BrdU阳性标记率为(19.5± 3.2 ) % ;对照组为 (10 .5± 1.4 ) % ,两组具有显著性差异 (P <0 .0 1)。同时 ,Westernblot方法测定结果表明bFGF刺激后 ,海马成年神经前体细胞磷酸化ERK1/ 2活性升高 ,15min时为峰值 ,6 0min后恢复到基础水平。结论　bFGF通过激活ERK1/ 2信号转导途径促进成年海马神经前体细胞胞核内DNA的合成 。Objective To observed the effect of basic fibroblast growth factor(bFGF)on the cell proliferation and the activity of ERK1/2 in cultured hippocampus neural precursor cells from adult rat.Methods The pretreated cultured hippocampus neural precursor cells from adult rat in vitro were incubated with bFGF(20 ng/ml) and BrdU(10 M),then the synthesize of DNA and the activity of ERK1/2 were respectively investigated by flow cytometry system(FCM)and Western blotting.Results The result of FCM showed that the bFGF increased the BrdU positive cell labeling index in cultured hippocampus neural precursor cells from adult rat in vitro as compared with control(P<0.01).At the same time. Western blotting also showed that the activity of phosphor ERK1/2 were enhanced markedly and reached the peak at the 15 min and return the baseline at 60 min after adding bFGF.Conclusion bFGF can enhance the DNA synthesize and the cell proliferation in cultured hippocampus neural precursor cells from adult rat in vitro by activated the signal transaction path of ERK1/2.

关 键 词：碱性成纤维细胞生长因子 神经前体细胞 细胞外信号调节蛋白激酶 信号转导 大鼠 动物 实验 

分 类 号：R338.26[医药卫生—人体生理学] R-332[医药卫生—基础医学]