异丙酚对缺氧复氧后大鼠海马神经元诱生型一氧化氮合酶表达的影响  

Effects of propofol on expression of inducible nitric oxide synthase in hippocampal neurons after anoxia-reoxygenation

作　　者：刘荣国[1] 何爱霞[1] 李立环[1] 

机构地区：[1]中国医学科学院阜外心血管病医院麻醉科,北京市100037

出　　处：《中华麻醉学杂志》2004年第5期365-368,共4页Chinese Journal of Anesthesiology

摘　　要：目的 观察异丙酚对原代培养海马神经元缺氧复氧后诱生型一氧化氮合酶(iNOS)表达及存活率的影响。方法 原代培养SD大鼠海马神经元随机分为四组:正常对照组、缺氧组、缺氧+异丙酚4μg/ml组、缺氧+异丙酚12μg/ml组。MTT法测定体外缺氧4h复氧24h后海马神经元的存活率,免疫细胞化学法测定iNOS表达的程度。结果 与缺氧组比较,两异丙酚组缺氧复氧后海马神经元的存活率提高(P<0.05),iNOS表达的灰度值降低(P<0.05或0.01),iNOS阳性表达率降低,并呈剂量依赖性(P<0.01)。结论 异丙酚可降低大鼠海马神经元缺氧复氧后iNOS的表达,提高存活率。Objective To investigate the effects of propofol on the survival rate of and the expression of inducible nitric oxide synthase (iNOS) in the cultured hippocampal neurons.Methods Hippocampal neurons isolated from new-born SD rats were dispersed and cultured in B27 culture medium for 9-10days.The cultured hippocampal neurons were randomized to one of 4 groups: (1)control group; (2) anoxia group; (3)propofol 4μg· ml^(1) + anoxia group; (4) propofol 12 μg·ml^(-1)+ anoxia group. For anoxia the cultured hippocampal neurons were placed in a tightly closed vessel filled with 95 % N_2～5% CO_2 at 37℃ for 4h,followed by 24 h reoxygenation. In the propofol groups (group 3 and 4) propofol was added before anoxia. Survival rates were measured by MTT assay The levels of iNOS expression were determined by immunocytochemistry method.Results In the two propofol groups (group 3 and 4) propofol attenuated the levels of iNOS expression in the cultured hippocampal neurons and increased the survival rates as compared with anoxia group (group 2). Depofol reduced the levels of iNOS expression in a concentration-dependent manner.Conclusion Propofol can decrease the level of iNOS expression in cultured hippocampal neurons induced by anoxia-reoxygenation and increase the survival rate of hippocampal neuron after anoxia-reoxygenation. Inhibition of iNOS expression may explain partly the mechanism of cerebral protective effects of propofol.

关 键 词：异丙酚 缺氧复氧 大鼠 海马神经元诱生型 一氧化氮合酶 二异丙酚 INOS 

分 类 号：R96[医药卫生—药理学]