两种生长因子对犬环杓后肌神经再支配的作用  

Effect of growth factor on nerve regeneration of the posterior cricoarytenoid muscle in dogs

作　　者：温武[1] 余永伟[2] 周水淼[1] 崔毅[3] 杨心青[4] 章如新[1] 张青[1] 

机构地区：[1]上海第二军医大学长海医院耳鼻咽喉科,200433 [2]长海医院病理科 [3]长海医院肌电图室 [4]福建省立医院耳鼻咽喉科

出　　处：《中华显微外科杂志》2004年第2期123-125,i005,共4页Chinese Journal of Microsurgery

基　　金：国家自然科学基金资助项目 (3970 0 1 56)

摘　　要：目的　研究碱性成纤维细胞生长因子 (bFGF)和神经生长因子 (NGF)在犬环杓后肌神经肌蒂移植术中的促神经再生作用。　方法　在切断犬喉返神经后 ,对失神经环杓后肌行颈袢神经胸骨甲状肌蒂移植术 ,并在局部给予bFGF、NGF及纤维蛋白凝胶 (FG) ,通过喉镜、神经电生理合检测、肌收缩强度测定及组织化学检查评价神经再支配情况。　结果　术后 6个月 ,喉镜查见联合组 (bFGF加NGF加FG组 )术侧声带深吸气时外展最明显 ,幅度接近健侧 ;NGF组 (NGF加FG)次之 ,对照组 (FG)最差 ;神经电生理指标、肌收缩强度依联合组、NGF组、对照组顺序下降 ,差异有显著性 (P <0 0 5或P <0 0 1)。组织学检查前两组运动终板分布较均一 ,肌纤维结构正常 ;对照组运动终板分布不均一 ,肌纤维粗细不均。　结论　bFGF和NGF在失神经环杓后肌神经肌蒂移植术中有显著促神经再生作用。且两者有协同作用。Objective To investigate the effects of basic fibroblast growth factor (bFGF) and nerve growth factor (NGF) on nerve regeneration of the posterior cricoarytenoid muscles (PCM) in dogs. Methods After the laryngeal recurrent nerves transected,the denervated PCM were reinnervated by nerve-muscle pedicle implantation in 15 dogs.The animals were divided into 3 groups randomly:bFGF and NGF and fibrin glue (FG) group (bFGF+NGF+FG),NGF group (NGF+FG) and control group (FG),Functional recovery of PCM was observed in by laryngoscope.Electrophysiological acvitity and muscle contract strength were determened.Histochemical studies were demonstrated by Kamovsky and HE stain. Ruselts After 6 months of operation,functional recovery and nerve regeneration of the PCA in bFGF+NGF+FG group were the best in 3 groups,which were closed to normal,and functional recovery in NGF group was better than that in control group.The parameters of electrophysiological acvitity and muscle contract strength were different in bFGF+NGF+FG group,NGF group,and control group ( P <0.05 or P <0.01). Conclusion FG is prior to suture in nerve-muscle pedicle implantation.bFGF and NGF can significantly promote the nerve regenertion of the PCA after denervation,and NGF can cooperate with bFGF in nerve regeneration.

关 键 词：犬 环杓后肌神经 BFGF 碱性成纤维细胞生长因子 神经生长因子 NGF 

分 类 号：R767.91[医药卫生—耳鼻咽喉科]