变形链球菌SBR经颌下腺免疫小鼠后局部神经生长因子和白介素-5表达变化  

Promotions of NGF and IL-5 mRNA Expression in Submandibular Glands of Mice after Vaccination with SBR Subunit Vaccine

作　　者：卢志山[1] 姜广水[1] 吴益华[2] 刘浩[1] 孙继军[1] 

机构地区：[1]山东大学口腔医学院口腔内科,济南250012 [2]济南市口腔医院牙体牙髓科,济南250001

出　　处：《口腔医学》2004年第3期129-131,共3页Stomatology

基　　金：国家自然科学基金项目 ( 3 0 1710 10 )

摘　　要：目的　探讨在涎腺免疫接种后局部免疫调节相关细胞因子的变化。方法　以变形链球菌SBR作为亚单位疫苗经颌下腺注射免疫BALB/C小鼠 ,应用逆转录 -聚合酶链反应 (RT PCR)检测接种后颌下腺内神经生长因子和白介素 5的表达。结果　免疫接种之后 ,小鼠颌下腺内神经生长因子和白介素 5的mRNA表达量均高于对照组。结论　变形链球菌SBR在涎腺作局部免疫接种 ,可诱导抗体应答 ,促进免疫调节相关细胞因子的表达 ,进一步增强涎腺的免疫功能和分泌功能。Objective To study changes of related cytokine mRNA expression in submandibular glands of mice after vaccination with streptococcus mutans SBR as subunit vaccine. Methods After vaccination of submandibular glands with SBR subunit vaccine, the author used RT-PCR to examine NGF and IL-5 mRNA expression in submandibular glands of mice. Results Levels of both NGF mRNA and IL-5 mRNA were higher in the immunized mice than those in the controls. Conclusion Vaccination of submandibular glands with streptococcus mutans SBR subunit vaccine could not only induce immune response but also enhance the expression of related cytokines which could further promote secretive function of specific antibody, and then could improve the effectiveness of anticaries.

关 键 词：变形链球菌 颌下腺 白介素-5 神经生长因子 

分 类 号：R349.5[医药卫生—基础医学]