凝血因子Ⅶ基因Arg353Gln多态性、活性和冠状动脉病变的关系  

Study on the relation of the Arg353Gln polymorphism of FⅦ gene and,the FⅦ activity to the severity of coronary lesions

作　　者：陶蓉[1] 于金德[1] 陆林[1] 乐玮[1] 尤蓓[1] 龚兰生[1] 

机构地区：[1]上海第二医科大学附属瑞金医院心内科,上海200025

出　　处：《诊断学理论与实践》2004年第3期203-205,共3页Journal of Diagnostics Concepts & Practice

摘　　要：目的:探讨不同严重程度的冠状动脉病变与凝血因子Ⅶ(FⅦ)活性及其Arg353Gln多态性的关系。方法:运用聚合酶链反应(PCR)、变性梯度胶电泳(DGGE)方法筛查123例冠心病患者和120例正常对照者FⅦ基因Arg353Gln多态性,以一期法检测研究对象FⅦ活性,分析冠状动脉病变、FⅦ基因Arg353Gln多态性和FⅦ活性之间的关系。结果:发现F基因8号外显子Arg353Gln多态性(等位基因M1/M2)与FⅦ活性显著相关(P<0.05),等位基因M2与低FⅦ活性有关。冠脉多支病变患者FⅦ活性显著高于正常对照者(P<0.05)。结论:FⅦ基因Arg353Gln多态性可能是影响FⅦ活性的重要遗传标志,FⅦ活性增高可能是引起冠状动脉严重病变的原因之一。Objective To explore the relation of the Arg353Gln polymorphism of coagulation jnjnfactor Ⅶ (FⅦ) gene and FⅦ activity to the severity of coronary lesions. Methods FⅦ gene was screened by PCR-DGGE in 120 patients with coronary heart disease (CHD) and 123 controls. The plasma FⅦ activity (Fc) was examined by one stage clotting assay. Results The polymorphism of Arg353Gln in exon 8 (allele M1, M2) of FⅦ gene was associated with the plasma FⅦc level. The allele M2 was related to low plasma FⅦc level (P<0.05). The plasma FⅦc in the patients with the multi-vascular lesions of coronary arteries was significantly higher than that in controls (P<0.05). Conclusion Arg353Gln polymorphism of FⅦ gene might be an important genetic mark to affect FⅦc. The raised FⅦc may be one of the potential risk factors of the multi-vascular lesions of coronary arteries.

关 键 词：冠状动脉病变 凝血因子Ⅶ 基因 多态性 活性 聚合酶链反应 变性梯度胶电泳 

分 类 号：R541.4[医药卫生—心血管疾病] Q781[医药卫生—内科学]