流动相添加剂法与手性固定相法对己唑醇光学异构体的拆分  

Chiral Separation of Hexaconazole by Reversed Phase HPLC with β-Cyclodextrin as a Mobile Phase and Normal Phase HPLC with CDMPC as Chiral Stationary Phase

作　　者：杨丽[1] 江树人[1] 廖勇[2] 王鹏[1] 田芹[1] 周志强[1] 

机构地区：[1]中国农业大学应用化学系,北京100094 [2]中国科学院遗传与发育生物学研究所,北京100101

出　　处：《农药学学报》2004年第2期90-92,共3页Chinese Journal of Pesticide Science

摘　　要：分别采用β-环糊精作为手性流动相添加剂的反相高效液相色谱法和涂敷型纤维素-三(3,5-二甲基苯基氨基甲酸酯)正相高效液相色谱手性固定相法拆分了农药己唑醇。结果表明,己唑醇对映异构体在以上两种方法下都完全达到基线分离。考察了影响容量因子、选择性系数和分离度的各种因素。Chiral separation of hexaconazole enantiomers was realized by reversed phase HPLC with β-cyclodextrin as a mobile phase and normal phase HPLC with CDMPC as chiral stationary phase. With reversed phase HPLC, the effect of the content of β-CD, pH of mobile phase and (organic) modifier on the chiral separation was studied. And the effect of 2-propanol concentration on the retention and resolution of CDMPC was investigated also. The results show that excellent enantioseparation can be achieved for hexaconazole by these two methods under the (optimal) (conditions.)

关 键 词：高效液相色谱 手性固定相 手性流动相添加剂 己唑醇 对映体拆分 

分 类 号：O657.72[理学—分析化学]