增敏^(89)Sr内照射时小鼠骨髓DNA损伤的实验研究  

Influence of ^(89)Sr therapy with radiosensitizer on DNA damage of bone marrow in mice

作　　者：李体远[1] 张英男[2] 黄瑞芳[1] 郑向鹏[2] 郭一玲[2] 顾望为[3] 林继红[3] 

机构地区：[1]暨南大学医学院附属第二医院深圳市人民医院临床医学研究中心,深圳518020 [2]暨南大学医学院附属第二医院深圳市人民医院核医学科,深圳518020 [3]第一军医大学实验动物中心

出　　处：《中华放射医学与防护杂志》2004年第3期246-248,共3页Chinese Journal of Radiological Medicine and Protection

基　　金：广东省自然科学基金资助项目 ( 0 0 0 3 72 )

摘　　要：目的　探讨89Sr内照射治疗时增敏剂烟酰胺和Carbogen对小鼠骨髓网织红细胞DNA损伤的影响。方法　昆明系小鼠 ,按组注射烟酰胺和 (或 )吸入气体Carbogen ,对照组注射同体积生理盐水。经小鼠尾静脉“弹丸”注射 2 0 0 μl89SrCl,放射性活度 74 0 0kBq( 2 0 0 μCi)。正常组注射同体积生理盐水。分别于注射后第 1,2 ,3,4 ,6 ,8,15 ,2 0 ,30 ,6 0和 90天批量处死小鼠 ,立即分离股骨骨髓 ,荧光法进行小鼠骨髓网织红细胞微核测定。结果　治疗组微核出现频率随观察时间呈双峰 ,各组第 1峰出现时间在第 2～ 4天 ,第 2峰在第 10～ 14天。阴性对照和阳性对照组比较 ,微核产生频率差异有显著性 (F =15 5 17,P <0 0 0 1)。其余各组 ( 89Sr+N +C ,89Sr,89Sr+N和89Sr+C)间相比较差异无显著性 (F =0 717,P >0 0 5 )。与89Sr组比较 ,单微核、双微核及三微核形成率差异均无显著性。未观察到四微核细胞。Objective To study the influence of 89 Sr therapy with radiosensitizer on DNA damage of bone marrow in mice. Methods Chinese Kunming mice were divided into 5 groups respectively:negative control (saline),positive control ( 89 Sr), 89 Sr+nicotinamide, 89 Sr+Carbogen and 89 Sr+nicotinamide+carbogen groups. 89 SrCl was intravenously injected as a blous containing activites of 7400 kBq(200 μCi)/200μl and saline was administered in equal volume in the negative control group.On days 1,2,3,4,6,8,15,20,30,60 and 90 after injection,mice were sacrificed for micronuclei assay in femoral marrow reticulocytes (MnRETs).One thousand randomly selected reticulocytes from each mouse were scored for the number of micronuclei. Results The average frequency of MnRETs induced as a function of time after injection is shown in a dual peak curve.The first maximum MnRETs frequency occurred between the 2 nd and the 4 th day,and the second between the 10 th and the 14 th day.A significant statistic difference on the MnRETs frequency was found between the negative and the positive control groups( P< 0 001),while no differences were noted among 89 Sr+N+C, 89 Sr, 89 Sr+N and 89 Sr+C groups ( P >0 05).There were also no significant differences on single,double or ternary micronuclei cell frequencies among 89 Sr+N+C, 89 Sr, 89 Sr+N and 89 Sr+C groups ( P> 0^05).No tetrad microncuclei cell was found in the experiment. Conclusion Administration of radiosensitizer does not aggravate the toxicity on bone marrow.

关 键 词：增敏^89Sr内照射 小鼠 骨髓DNA损伤 CARBOGEN 增敏剂 

分 类 号：R817[医药卫生—影像医学与核医学]