精子运动能力与一氧化氮关系的探讨  

Relationship between sperm movement and nitric oxide in sperm

作　　者：杨麦贵[1] 同丽萍[2] 张竹映[3] 郑善銮[1] 钟丽辉[1] 皇海[1] 徐焰[1] 樊新[1] 刘田[1] 

机构地区：[1]第四军医大学西京医院检验科,西安710032 [2]第四军医大学西京医院药剂科,西安710032 [3]中国铁道建筑总公司西安医院

出　　处：《中国优生与遗传杂志》2004年第3期110-111,共2页Chinese Journal of Birth Health & Heredity

摘　　要：目的　探讨一氧化氮 (NO)与精子运动能力的关系。方法　采用镀铜镉还原荧光法 ,测定人精液中NO代谢产物硝酸盐 (NO3 -)。参照WHO方法 ,在超高倍显微镜下观察精子存活率、活动力等。结果　 80例男性不育者其中 15例NO浓度明显低于正常对照组精子活动力a +b级 <5 0 % (P <0 .0 1) ,以运动轨迹异常和头摆动幅度下降为主 ,精子存活力 >75 %无明显差异 (P >0 .0 5 ) ;6 5例NO浓度显著高于正常对照精子活动力a +b级 <5 0 % ,存活率 <75 % (P <0 .0 0 1)。结论　NO与精子运动功能有着密切关系。高浓度时明显抑制精子活动力及存活率。低浓度时精子存活率及功能有着维护作用 ,这对男性不育的病因研究和治疗有非常重要的临床价值。Objective: To study the relationship between Sperm movement and nitric oxide in sperm. Methods: Fluorescence reducibility by copper-coated cadmium was used to detect nitrate from metabolism of nitric oxide in sperm. Results: In 80 sperm samples,there were 65 samples that concentration of nitric oxide is higher than hardly that in control group which activity grade a+b is <50% and livability is <75% ( P <0.001). Conclusion: There was significant relationship between sterility and nitric oxide in sperm.

关 键 词：一氧化氯 男性不育 精子运动功能 

分 类 号：R3211[医药卫生—人体解剖和组织胚胎学]