梅毒螺旋体TpN15抗原基因的扩增、克隆及表达  

Cloning and expression of Recombinant treponema pallidum TpN15 antigen with genetic engineering technique

作　　者：陈黎明[1] 李克深[1] 李岫嵘[1] 赵悦[1] 

机构地区：[1]解放军第211医院优生优育中心,哈尔滨150080

出　　处：《中国优生与遗传杂志》2004年第3期18-19,共2页Chinese Journal of Birth Health & Heredity

摘　　要：目的　用基因工程技术表达梅毒螺旋体TpN15重组抗原 ,为进一步研制梅毒预防性疫苗和诊断试剂盒打下基础。方法　采用PCR技术扩增梅毒螺旋体TpN15基因 ,然后克隆到原核表达载体pET2 8b中表达TpN15重组抗原蛋白。结果　成功的构建了pET2 8b -TpN15重组表达载体 ,重组的TpN15蛋白在大肠杆菌BL2 1中得到稳定表达。结论　梅毒螺旋体TpN15基因在大肠杆菌中的成功表达为建立梅毒血清学诊断的新方法奠定了基础。Objective: To produce recombinant TpN15 Treponema pallidum antigen with genetic engineering 1technique. Methods: The TpN15 gene was amplified from TP genome by PCR and cloned into prokaryotic expression vector to express recombinant TpN15 protein. Results:The expression vector pET28b-TpN15 was constructed successfully, and TpN15 protein was highly expressed in E .coli BL21. Conclusion The acquirement of recombinant TpN15 protein lay a foundation for new clinical diagnosis method of syphilis.

关 键 词：梅毒螺旋体 基因表达 重组抗原 

分 类 号：R392[医药卫生—免疫学]