微量血提取DNA法在PPARγ基因突变研究中的应用  

Extract DNA from micro blood samples in the study of mutation in the PPARγ gene

作　　者：张德峰[1] 朱俊真[1] 高健[1] 郭文潮[1] 郝玉宾[1] 

机构地区：[1]河北省人民医院优生优育优教技术中心,050071

出　　处：《中国优生与遗传杂志》2004年第3期26-27,共2页Chinese Journal of Birth Health & Heredity

摘　　要：目的　建立一种大样本外周血基因组DNA的提取方法。方法　改良经典基因组DNA提取方法 ,用TritonX -10 0和Tris-HCl混合溶解红细胞。结果　用该法提取的DNA ,A2 60nm/A2 80nm均大于 1.89,含量大约 30 0 μg/ml,PCR扩增产物酶切结果良好。结论　该方法简便、经济 ,可用于大样本的基因分析。Objective: To establish one kind of method of genomic DNA extraction for numbers of samples. Methods: We improved the traditional method of extract DNA by using the mixture of Triton-X and Tris-HCl to lyses RBC. Results: DNA extracted with this method was about 300μg/ml. A 260 /A 280 was greater than 1.89, and the result of restriction enzyme digesting the amplified PCR products was good. Conclusion: The method is simple, economical and could be used for numbers of samples' gene analysis.

关 键 词：基因组DNA 大样本 PCR 

分 类 号：R346[医药卫生—基础医学]