HPLC法测定脑脉通口服液中黄芪甲苷的含量  

Determination of content of astragaloside Ⅳ in Naomaitong koufuye by HPLC method

作　　者：沈阳[1] 沈利明[1] 吴兴文[2] 舒薇[2] 

机构地区：[1]第二军医大学长征医院药学部,上海200003 [2]第二军医大学长征医院闸北分院药材科,上海200071

出　　处：《药学服务与研究》2004年第2期163-164,共2页Pharmaceutical Care and Research

摘　　要：目的 :建立脑脉通口服液中黄芪甲苷的含量测定方法 ,控制药品质量。 方法 :采用HPLC法 ,反相柱YWG C18为固定相 ,以乙腈 0 .15 %磷酸溶液 (1∶2 )为流动相 ,UV检测波长为 2 0 5nm。 结果 :脑脉通口服液中黄芪甲苷的含量为 1.2 8mg/ml,平均回收率为 95 .4 % ,RSD为 1.4 7%。线性范围为 0 .2～ 8.0mg/ml,回归方程为Y =310 4 6 +6 982 3X ,r=0 .9996。 结论 :该方法简便灵敏 ,结果准确 ,能满足控制脑脉通口服液质量的要求。Objective: To determine the content of astragaloside Ⅳ in Naomaitong koufuye. Methods: The HPLC method was applied. The YWG-C 18 column was applied and a mixture of acetonitrile-0.15% phosphoric acid (1∶2) was used as the mobile phase. Astragaloside Ⅳ was detected at 205 nm. Results: The concentration of astragaloside Ⅳ in Naomaitong koufuye was 1.28 mg/ml. The average recovery rate was 95.4%, and the RSD was 1.47%. The linear range was 0.2-8.0 mg/ml. The regression equation was Y=31 046+ 69 823 X, r=0.999 6.Conclusion: The method is simple, sensitive and accurate. It can be used for quality control of Naomaitong koufuye.

关 键 词：脑脉通口服液 黄芪甲苷 色谱法 高压液相 

分 类 号：R286.26[医药卫生—中药学]