小鼠颈部心脏移植模型的改进  

Improved technique of cervical heterotopic heart transplantation in mice

作　　者：高思海[1] 李平[1] 潘铁成[1] 杨辰垣[2] 

机构地区：[1]华中科技大学同济医学院附属同济医院胸心外科,武汉430030 [2]华中科技大学同济医学院附属协和医院心外科,武汉430022

出　　处：《广东医学》2004年第7期756-757,共2页Guangdong Medical Journal

基　　金：国家自然科学基金资助项目 (编号 :3 9670 72 0 )

摘　　要：目的　探讨小鼠颈部心脏移植模型的改进和研究心脏移植再灌注损伤及急性排异反应。方法　将Chen氏术式加以改进 ,将供心主动脉与受体颈总动脉行端侧吻合 ,进行 6 0次小鼠颈部心脏移植。结果　新方法使供心总缺血时间缩短至 30min ,7d存活率提高到 98%。结论　改进后的模型简单实用 ,提高了小鼠颈部心脏移植存活率 ,值得推广。Objective To improve the technique of mouse cervical heterotopic heart transplantation and to study cardiac transplantation rejection and reperfusion injury. Methods By modifying Chen's method, the donor heart aorta and the right common carotid artery of the recipient were anastomosed using the end-to- side suture technique. Results The total ischeamic time of the graft shortened to 30 min and the 7 day survival rate of the recipient increased to 98% by using the new technique. Conclusions The development of cervical heart transplantation in mice is a novel, simple and useful technique.

关 键 词：小鼠 颈部心脏移植 动物模型 再灌注损伤 急性排异反应 

分 类 号：R654.2[医药卫生—外科学] R-33[医药卫生—临床医学]