Characteristics of photosynthesis in rice plants transformed with an antisense Rubisco activase gene  

作　　者：金松恒 蒋德安 李雪芹 孙骏威 

机构地区：[1]StateKeyLabofPlantPhysiologyandBiochemistry,CollegeofLifeSciences,ZhejiangUniversity,Hangzhou310029,China

出　　处：《Journal of Zhejiang University Science》2004年第8期897-899,共3页浙江大学学报（自然科学英文版）

基　　金：Project supported by the National Natural Science Foundation ofChina (No. 39970440); Doctoral Foundation of Ministry of Edu-cation of China (No. 20020335043) and the National Basic Re-search Program (973) of China (No. G1999011706)

摘　　要：Transgenic rice plants with an antisense gene inserted via Agrobacterium tumefaciens were used to explore the impact of the reduction of Rubisco activase (RCA) on Rubisco and photosynthesis. In this study, transformants containing 15% to 35% wild type Rubisco activase were selected, which could survive in ambient CO2 concentration but grew slowly compared with wild type controls. Gas exchange measurements indicated that the rate of photosynthesis decreased sig- nificantly, while stomatal conductance and transpiration rate did not change; and that the intercellular CO2 concentration even increased. Rubisco determination showed that these plants had approximately twice as much Rubisco as the wild types, although they showed 70% lower rate of photosynthesis, which was likely an acclimation response to the reduction in Rubsico activase and/or the reduction in carbamylation.Transgenic rice plants with an antisense gene inserted via Agrobacterium tumefaciens were used to explore the impact of the reduction of Rubisco activase (RCA) on Rubisco and photosynthesis. In this study, transformants containing 15% to 35% wild type Rubisco activase were selected, which could survive in ambient CO2 concentration but grew slowly compared with wild type controls. Gas exchange measurements indicated that the rate of photosynthesis decreased sig-nificantly, while stomatal conductance and transpiration rate did not change; and that the intercellular CO2 concentration even increased. Rubisco determination showed that these plants had approximately twice as much Rubisco as the wild types,although they showed 70% lower rate of photosynthesis, whichRubsico activase and/or the reduction in carbamylation.was likely an acclimation response to the reduction in Rubsico activase and/or the reduction in carbamylation.

关 键 词：Antisense rca rice plants RUBISCO Rubisco activase PHOTOSYNTHESIS 

分 类 号：S511[农业科学—作物学] Q945.11[生物学—植物学]