银屑病皮损细胞凋亡的研究  

Study on Cell Apoptosis in Psoriasis

作　　者：徐娟[1] 贾随旺 钟绮丽[3] 杨慧倩[1] 郑茂荣[4] 顾军[4] 

机构地区：[1]广东省深圳福田人民医院,广东深圳518033 [2]广东省深圳市人民医院,广东深圳518000 [3]北京大学深圳医院,广东深圳518000 [4]上海长海医院,上海200000

出　　处：《岭南皮肤性病科杂志》2004年第2期91-93,100,共4页Southern China Journal of Dermato-Venereology

摘　　要：目的 :通过对银屑病皮损细胞凋亡以及凋亡相关基因bcl 2、Fas、bax和Fas L、穿孔素的检测 ,探讨细胞凋亡与银屑病的关系。方法 :采用原位末端标记及免疫组化ABC法、SABC法。结果 :银屑病中细胞凋亡较正常人增多。抑凋亡基因bcl 2几乎不表达 ,而促凋亡基因Fas、bax阳性表达。细胞毒性T淋巴细胞可能通过Fas L和穿孔素两条途径起作用。结论 :细胞凋亡在银屑病发病机理中有十分重要的意义。Objective:To explore the relationship between cell apoptosis and psoriasis. Methods:Apoptosis, apoptotis related gene bcl-2, Fas, bax, Fas-L and perforin were detected by TUNEL, Immunohistochemical ABC and SABC methods. Results:In skin lesions of psoriasis, the suppressing apoptosis gene bcl-2 scarcely revealed positive result. But promoting apoptosis gene Fas and bax revealed positive reactivities. Cytotoxic T lymphocytes probably played role through Fas-L and perforin. Conclusion:The apoptosis was very important in pathogenesis of psoriasis.

关 键 词：银屑病 细胞凋亡 原位末端标记 穿孔素 

分 类 号：R758.63[医药卫生—皮肤病学与性病学]