A EPC Approach to the Residual Water in Erythrocytes  

作　　者：ZHAOGang GUOXiao-jie HELi-qun LIUZhong GAODa-yong 

机构地区：[1]InstituteofCryobiological＆MedicalEngineering,DepartmentofThermalScienceandEnergyEngineering,Hefei230027,P.R.China [2]RedCrossandBloodCenterofHefei,Hefei230022,P.R.China [3]DepartmentofMechanicalEngineering.UniversityofKentucky,Lexington,KY40506-0108,USA

出　　处：《Chinese Journal of Biomedical Engineering(English Edition)》2004年第1期21-27,共7页中国生物医学工程学报（英文版）

基　　金：This research is supported by NSFC(5 0 10 6 0 16 ) ,NSF of Anhui Province(0 0 0 4 75 2 0 ,0 30 4 3717)

摘　　要：A novel approach has been developed to determine the amount of residual water in human erythrocyte at room temperature by electronic particle counter. Nacl solutions of 13 osmolalities were prepared and the equilibrium cell volumes in which were measured one by one.The isotonic volume, V0, was obtained under the isotonic condition. The mean RBC volumes of 5 donors at each osmolality were fitted according to Boyle van’t Hoff relationship, and the osmotically inactive volume, Vb, of erythrocyte was then determined. The results show that Vb50% V0. More importantly, the final cell volume with regard to the solution of the highest concentration found to be kept at about 0.5 V0. The difference between these two volumes is unconspicuous. According to the published data that non-water volume of human erythrocyte is about 28.3% of its isotonic volume, residual water of human erythrocyte can be gained by subtracting V dry from Vf, that is V rw =21.7% V0. Then it was concluded that the residual water of human lays in 2 states, one is bound water, and the other is free water.A novel approach has been developed to determine the amount of residual water in human erythrocyte at room temperature by electronic particle counter. Nacl solutions of 13 osmolalities were prepared and the equilibrium cell volumes in which were measured one by one.The isotonic volume, V_0, was obtained under the isotonic condition. The mean RBC volumes of 5 donors at each osmolality were fitted according to Boyle van't Hoff relationship, and the osmotically inactive volume, V_b, of erythrocyte was then determined. The results show that V_b50% V_0. More importantly, the final cell volume with regard to the solution of the highest concentration found to be kept at about 0.5 V_0. The difference between these two volumes is unconspicuous. According to the published data that non-water volume of human erythrocyte is about 28.3% of its isotonic volume, residual water of human erythrocyte can be gained by subtracting V_ dry from V_f, that is V_ rw =21.7% V_0. Then it was concluded that the residual water of human lays in 2 states, one is bound water, and the other is free water.

关 键 词：Erythrocyte/RBC EPC OSMOLALITY Cell volume Boyle van't hoff 

分 类 号：R446.11[医药卫生—诊断学]