呆聪液对老龄痴呆动物模型影响的研究  

Effect of daicong solution on dementia models of aged rats

作　　者：张太国[1] 赵淑梅[2] 岳启安[2] 阎乐法[3] 管福来[2] 李光宗[4] 

机构地区：[1]潍坊医学院保健科,山东省潍坊市261042 [2]潍坊医学院免疫与病原生物学教研室,山东省潍坊市261042 [3]潍坊医学院中医教研室,山东省潍坊市261042 [4]潍坊医学院解剖学教研室,山东省潍坊市261042

出　　处：《中国临床康复》2004年第19期3837-3839,共3页Chinese Journal of Clinical Rehabilitation

基　　金：山东省中医管理局中医科技发展基金资助(98024)~~

摘　　要：目的:探讨呆聪液对老龄痴呆大鼠动物模型脑M受体、某些血清酶活性与过氧化脂质(lipidperoxides,LPO)及免疫功能的影响。方法:采用双侧电解损毁22月龄大鼠脑基底核法,造成动物学习与记忆功能障碍的老龄鼠痴呆动物模型,用中药呆聪液灌胃给药1个月后,处死动物测定脑组织M受体;检测血清中超氧化物歧化酶(superoxidedismutase,SOD)、胆碱酯酶和单胺氧化酶(monoamineoxidase,MAO)的活性水平及LPO的含量;用3H-TdR掺入DNA法测定淋巴细胞增殖能力。结果:呆聪液可提高老龄痴呆大鼠脑M受体数目(P<0.01);提高血清SOD活性:呆聪液10g/(kg·d)组,SOD活性为(173.02±23.07)NU/mL,与模型组(75.75±11.21)NU/mL比较,差异有非常显著性意义(q=15.13,P<0.01)。呆聪液可降低LPO含量(P<0.01),降低MAO和胆碱酯酶活性(P<0.01);提高淋巴细胞增殖能力(P<0.01)。结论:呆聪液明显提高老龄痴呆大鼠脑M受体数目,对氧自由基具有一定的清除作用,还可清除LPO和抑制胆碱酯酶与MAO的活性,具有益智抗衰老及提高免疫功能的作用。对老年性痴呆有较好的疗效。AIM:To explore the effect of daicong sloution on brain M receptor, some serum enzyme activity,lipid peroxides(LPO) and the immunity of aged dementia rat models. METHODS:22 month old rats whose basal nuclei were destroyed by bilateral electrolytic method were used as aged dementia rat models which had learning and memory disability.They were treated with daicong solution for a month.Then the rats were killed and the number of M receptors in the brain tissue and the activities of superoxide dismutase(SOD),cholinesterase(CHE) and monoamine oxidase(MAO),and LPO level in the serum were measured. RESULTS:Daicong solution could increase the number of M receptors in the brain tissue(P< 0.01)and the activity of SODEffect of daicong solution on dementia models of aged rats$$$$ Tai Guo Zhang1,Shu Mei Zhao2,Qi An Yue2,Le Fa Yan3,Fu Lai Guan2,Guang Zong Li4,1Department of Health,2Department of Immunity and Pathogenic Biology,3Department of Traditional Chinese Medicine,4Department of Anatomy, Weifang Medical College,Weifang 261042,Shandong Province,ChinaAbstract AIM:To explore the effect of daicong sloution on brain M receptor, some serum enzyme activity,lipid peroxides(LPO) and the immunity of aged dementia rat models. METHODS:22 month old rats whose basal nuclei were destroyed by bilateral electrolytic method were used as aged dementia rat models which had learning and memory disability.They were treated with daicong solution for a month.Then the rats were killed and the number of M receptors in the brain tissue and the activities of superoxide dismutase(SOD),cholinesterase(CHE) and monoamine oxidase(MAO),and LPO level in the serum were measured. RESULTS:Daicong solution could increase the number of M receptors in the brain tissue(P< 0.01)and the activity of SOD[(173.02±23.07) NU/mL for the daicong solution group(10 g/kg body mass per day) and(75.75±11.21) NU/mL for the model group, with significant difference(q= 15.13,P< 0.01),and decrease the content of LPO(P< 0.01) and activities of MAO and CHE(P< 0.01

关 键 词：呆聪液 老年痴呆 动物模型 超氧化物歧化酶 检测 胆碱酯酶 中医药疗法 

分 类 号：R277.791.6[医药卫生—中医学]