人外周血内皮祖细胞培养及特性  

Culture of human endothelial progenitor cells in peripheral blood and their characteristics

作　　者：许勤[1] 陈书艳[1] 金誉[1] 荣烨之[1] 

机构地区：[1]上海第二医科大学附属新华医院外科,200092

出　　处：《中华实验外科杂志》2004年第5期602-603,i004,共3页Chinese Journal of Experimental Surgery

基　　金：上海市科学技术发展基金资助项目 (0 1 4 1 1 90 91 )

摘　　要：目的　探讨人外周血来源的内皮祖细胞 (EPCs)体外诱导培养和扩增的方法 ,以期证实人外周血是临床治疗缺血性疾病一个理想的内皮祖细胞来源。方法　采集正常人外周血单个核细胞进行体外培养 ,应用免疫组织化学和免疫荧光技术进行内皮细胞表面标记物检测 ,应用细胞荧光化学检测进行培养细胞的内皮细胞功能检测。结果　人外周血单个核细胞 (MNCs)经体外培养 ,表达内皮细胞的特异性抗原 ,包括Ⅷ因子 (VWF)、FLK 1、CD3 1和CD3 4,并显示出能内吞乙酰低密度脂蛋白 (acLDL) ,结合UEA 1等内皮细胞的特性 ,提示这些培养细胞具有内皮细胞的表面蛋白特性和具有内皮细胞的功能。结论　人外周血中存在具有分化增殖能力的EPCs ,可体外扩增和收集。Objective To examine whether endothelial progenitor cells (EPCs) could be isolated from peripheral blood and could be cultured and proliferated in vitro.Methods Total hPBMCs were isolated from peripheral blood of human volunteers and were cultured in EC basal medium-2.Culture cells were subjected to immunofluorescence and immunocytochemistry to analyze the expression of VWF,FLK-1,CD31,CD34 and AC133.Fluoresencent chemical detection was performed to detect dual binding of UEA-1 and acLDL,consistent with endothelial lineage cells.Results Culture cells were positively stained for VWF,FLK-1,CD31,CD34 and AC133 and could be shown to endocytose UEA-1 and acLDL.Conclusion EPCs have been shown to be present in circulation,in quantities sufficient to permit their harvest and in vitro expansion.

关 键 词：外周血 内皮祖细胞 细胞培养 特性 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]