利用基因表达谱芯片研究东方田鼠和小鼠感染日本血吸虫前后基因的差异性表达  

Gene Difference Expression Between Mice and Microtus fortis Infected with Schistosoma japonicum Using cDNA Microarrays

作　　者：孙军[1] 林矫矫[1] 程国锋[1] 李浩[1] 石耀军[1] 陆珂[1] 蔡幼民[1] 江洪波[2] 

机构地区：[1]中国农科院上海家畜寄生虫病研究所,农业部动物寄生虫学重点实验室,上海动物生物技术研究中心,上海200232 [2]中国科学院上海生命科学信息中心,上海200031

出　　处：《北京大学学报（自然科学版）》2004年第4期532-537,共6页Acta Scientiarum Naturalium Universitatis Pekinensis

基　　金：上海科技发展基金项目 (2 0 0 1 4 90 90 0 2 );农业部动物寄生虫学重点试验室开放课题

摘　　要：利用大、小鼠基因表达谱芯片分别研究了Balb c小鼠感染日本血吸虫 7d时肺组织、东方田鼠感染日本血吸虫 7d时肺组织、1 2d时的肺和肝组织基因差异表达方式。结果表明 ,小鼠感染日本血吸虫 7d时肺丝氨酸蛋白酶抑制剂 (Serineproteaseinhibitor)基因表达上调 ,同时没有免疫相关基因的表达变化 ;与之相反 ,东方田鼠感染日本血吸虫 7d时肺丝氨酸蛋白酶抑制剂基因没有表达变化 ,非特异性免疫相关基因 ,如溶菌酶 (Lysozyme)和各类组织蛋白酶 (Cathepsin)基因表达上调 ,而且肺内特异性免疫相关基因如CD74、MHCⅡ和MHCⅠ等表达上调。这一现象得到东方田鼠感染日本血吸虫 1 2d时肺和肝中特异性免疫和非特异性免疫相关基因的上调表达的进一步证实。而且 ,东方田鼠感染日本血吸虫 1 2d时肺中丝氨酸蛋白酶抑制剂基因表达下调 ,肝组织中胰岛素样生长因子 1 (Insulin likegrowthfactor 1 ,IGF 1 )基因表达下调。提示日本血吸虫适宜性宿主小鼠和非适宜性宿主东方田鼠感染日本血吸虫前后两者有相反的基因表达方式。Gene difference expression of the lung tissues between of mice and Microtus fortis infected with Schistosoma japonicum for 7?d, and the lung tissues and the liver tissues of Microtus fortis infected with Schistosoma japonicum for 12?d were analyzed by cDNA microarrays. The results showed that serine protease inhibitor genes expression was up regulated and immune associated genes expression were not obviously changed in the lung tissues of mice. On the contrary, serine protease inhibitor genes expressed constantly in the lung tissues of Microtus fortis infected with Schistosoma japonicum for 7?d and Several non specific immune associated genes (such as lysozyme gene and cathepsin genes) were up regulated. At the same time, some important specific immune associated genes(such as CD74、MHC Ⅱ and MHC Ⅰ) were up regulated. The lung tissues and liver tissues of Microtus fortis infected with Schistosoma japonicum for 12?d also confirmed the results by enhancing expression of the non specific immune associated genes and specific immune associated genes. Furthermore, in the lung tissues of Microtus fortis infected with Schistosoma japonicum for 12?d serine protease inhibitor genes expression were down regulated. It was suggested that the mice and Microtus fortis share reverse expression patterns, which was helpful to deeply understand the molecular mechanism of natural resistance of Microtus fortis to Schistosoma japonicum .

关 键 词：东方田鼠 基因芯片 丝氨酸蛋白酶抑制剂 溶菌酶 组织蛋白酶 CD74 MHC Ⅱ MHC Ⅰ 

分 类 号：R383.24[医药卫生—医学寄生虫学]