发育中大鼠皮层神经元早期无镁细胞外液处理诱导NMDAR亚基mRNA表达的远期变化  

Long-Term Changes of NMDAR Subunit mRNA Expression Induced by Early Magnesium-Free Treatment in Developing Cortical Neurons of Rat

作　　者：王静敏[1] 姜玉武[1] 曹海燕[1] 薄涛[1] 吴希如[1] 

机构地区：[1]北京大学第一医院儿科,北京100034

出　　处：《北京大学学报（自然科学版）》2004年第4期550-556,共7页Acta Scientiarum Naturalium Universitatis Pekinensis

基　　金：国家自然科学基金 (30 2 70 4 4 8) ;北京大学国家"2 1 1工程" ;中国博士后科学基金 (2 0 0 30 330 82 )资助项目

摘　　要：以原代培养的大鼠大脑皮层神经元无镁诱导的反复惊厥样放电为模型 ,根据对培养 6d(天 )皮层神经元的不同处理分为 3组 :正常DMEM培养液组 (CONT1 )、正常细胞外液组 (CONT2 )和无镁细胞外液组 (MGF)。神经元在上述 3种液体中孵育 3h ,然后恢复正常DMEM培养液继续培养 ,在培养 7、1 2及 1 7d时应用实时定量PCR测定了发育中大鼠皮层神经元无镁细胞外液处理后NMDA受体亚基NR1、NR2A与NR2BmRNA表达的变化。结果显示 :MGF组NR1mRNA表达在培养 7d时明显降低 ,培养 1 2d时明显升高 (p <0 0 5 ) ;NR2AmRNA表达在培养 1 2d时明显降低 ,培养 1 7d时明显升高 (p <0 0 5 ) ;NR2BmRNA表达在培养 7d与 1 7d时明显升高 (p <0 0 5 )。而培养1 7dNR1mRNA表达、7dNR2AmRNA表达及 1 2d的皮层神经元NR2BmRNA表达 3组中两两比较无统计学差异 (p >0 0 5 )。MGF组NR1 NR2A、NR1 NR2B和NR1 NR2A NR2B在培养 7、1 2与 1 7d皮层神经元均有明显的变化。To explore long term changes of NMDAR subunit mRNA expression induced by early magnesium free treatment in developing cortical neurons of rat. The seizure model was recurrent epileptiform discharges induced by transient magnesium free media treatment in the primary cultured embryo cortical neurons of rat. According to different treatment to cortical neurons at cultured day 6, three groups were divided: regular DMEM medium (CONT1)、regular extracellular solution medium (CONT2) and magnesium free extracellular solution medium (MGF). The neurons were exposed to above three media for 3h in cultured day 6, and then cultured in regular DMEM medium again. The expression of NMDAR subunit NR1, NR2A ,and NR2B mRNA was measured using fluorescent real time quantitative RT PCR at different cultured day (7、12、17). Our results in group MGF showed that NR1 mRNA expression decreased at d7 and increased at d12 ( p <0 05), NR2A mRNA expression decreased at d12 and increased at d17 ( p <0 05), and NR2B mRNA expression increased at d7 and d17 ( p <0 05). There were not statistic significant differences of mRNA expression of NR1 at d17, NR2A at d7, and NR2B at d12 among three groups ( p >0 05). There were significant alterations of proportion of NR1/NR2A, NR1/NR2B and NR1/NR2A/NR2B mRNA expression at d7,d12 and d17 in group MGF. The change of NMDAR subunit mRNA expression may be resulted by long term changes of early magnesium free treatment in developing cortical neurons of rat.

关 键 词：惊厥 远期 N-甲基-D-天冬氨酸 受体 mRNA 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]