福氏2a痢疾杆菌膜抗原的免疫电镜定位  

Immunoelectron Microscopic Localization of the Membrane Antigens on the Cell Wall of Shigella Flexneri 2a

作　　者：李红[1] 周佳敏[1] 王国华[1] 高杰英[1] 陈德蕙[1] 

机构地区：[1]军事医学科学院

出　　处：《电子显微学报》1993年第5期373-376,共4页Journal of Chinese Electron Microscopy Society

摘　　要：本文报告应用抗福氏2a痢疾杆菌膜成份McAb及胶体金探针对细菌膜抗原进行了定位。结果显示4种抗脂多糖McAb(3A6、2E6、4F1、2A3)可不均一的识别位于细菌壁表面的抗原,而另外一种抗LPS的McAb种(1G8)和一种抗膜蛋白McAb(1A1)识别的抗原未暴露于细菌表面。应用细菌膜碎片包埋前染色成功定位了抗膜蛋白McAb(1A1)位于细菌内膜的抗原。与McAb的生物活性比较表明阳性标记细菌表面抗原的McAb的抗原与其阻段志贺氏菌接触性溶血试验和对小鼠的被动保护力密切相关。提示免疫电镜标记技术确定LPS抗原表位在细菌表面的可及性和拷贝数对构建和筛选痢疾工程菌苗具有重要意义。The localizations of Shigella flexneri 2a membrane antigens were determined by electron microscopy by us- ing six McAbs against Shigella flexneri 2a membrane compnents and immunogold labeling.Binding of gold pati- cles the McAbs on the wall of whole bacterial cells indicated that the antigens recognized by McAb 3A6,2E6, 4Fl and 2A3 were exposed on the cell surface,and those by McAb 1A1 and 1G8 were not.Labeling for McAb 1A1 was successfully performed by using sonicated bacterial sediment and pre-embeded immunogold staining, Gold particles on the inner membrane of the bacteria were observed.Comparing with the biological activities of these McAbs,the results showed that exposure of the antigenic determinents on the bacteria surface had a direct bearing on their blocking activities of the contact haemolysis and the passive protecting capacties in mice.All these suggested that understanding of the accessibility and numbers of copies to the bacterial protecting antigenic determinents had a great significance in the construction and selection of Shigella vaccine.

关 键 词：免疫 金标记 抗原表位 痢疾杆菌 LPS 免疫电镜 

分 类 号：R378.25[医药卫生—病原生物学]