XK469和阿霉素对H460细胞生长的影响及其机制  

Effect of XK469 and adriamycin on the growth of H460 cells in vitro and its mechanism

作　　者：凌朝辉[1] 杜华[2] 袁长青[1] 马树东[3] 郑莉[1] 丁振华[1] 

机构地区：[1]第一军医大学防原教研室 [2]第一军医大学教务处 [3]南方医院肿瘤科,广东广州510515

出　　处：《第一军医大学学报》2004年第7期775-778,共4页Journal of First Military Medical University

基　　金：广东省科技三项资助项目(2003C30105)~~

摘　　要：目的 探讨XK469和阿霉素对H460细胞的抑制作用及其机制。方法 采用MTT比色法观察不同浓度XK469、XN472和阿霉素对H460细胞生长的影响,采用流式细胞术观察药物作用下细胞周期的变化。采用Western blotting检测药物作用后H460细胞内cdc2和phos-cdc2的表达水平。结果 不同浓度XK469、阿霉素可明显抑制H460细胞的生长,导致H460细胞发生G2/M期阻滞,胞内phos-cdc2表达明显增高。20μg/ml以上浓度XN472作用24 h可抑制H460细胞生长,但是到120 h时抑制作用消失。结论 XK469和阿霉素通过使H460细胞内phos-cdc2水平增高导致其G2/M期阻滞从而抑制其增殖生长,7-氯结构对XK469的抗肿瘤效应有重要的意义。Objective To investigate the inhibitory effect of XK469 on the in vitro growth of H460 cells and its mechanism. Methods The survival curves of H460 cells treated with XK469, XN472 and adriamycin, respectively, were obtained by MTT analysis, and the effect of XK469 and adriamycin on the cell cycle of H460 cells examined by flow cytometry. Western blotting was adopted for detecting the expression of cdc2 and phos-cdc2 induced by XK469 and adriamycin. Results Different concentrations of XK469 and adriamycin could significantly inhibit the growth of H460 cells, induce their G2/M phase arrest, and increase phos-cdc2 expression; XN472 had a lesser effect on the growth of H460 cells. Conclusion XK469 can increase phos-cdc2 expression and induce G2/M phase cell cycle arrest of H460 cells, resulting in inhibition of H460 cell growth. The inhibitory effect of XK469 on H460 cell growth is attributed to the chlorine in the 7-positon of its structure.

关 键 词：XK469/投药和剂量 阿霉素/投药和剂量 细胞周期/药物作用 

分 类 号：R96[医药卫生—药理学]