胰岛素对大鼠动脉条分泌肾上腺髓质素的刺激作用  

Effect of insulin on the synthesis and secretion of adrenomedullin in rat aortas in vitro

作　　者：胥学伟[1] 唐朝枢[2] 杨晔[3] 李小鹰[1] 

机构地区：[1]解放军总医院南六科,北京市100853 [2]北京大学第一医院,北京市100034 [3]海军总医院,北京市100086

出　　处：《中国康复理论与实践》2004年第6期350-350,345,共2页Chinese Journal of Rehabilitation Theory and Practice

摘　　要：目的观察葡萄糖、胰岛素对大鼠动脉条合成与分泌肾上腺髓质素 (ADM )的作用。方法在孵育液中分别加入不同浓度的葡萄糖、胰岛素及葡萄糖 +胰岛素 ,用放免分析方法测定组织和孵育液中ADM的含量。结果与对照组比较 ,各组组织中ADM含量均无明显改变 (P >0 .0 5 ) ,孵育液中 ,葡萄糖各组和低浓度胰岛素组ADM的含量无显著性差异 (P >0 .0 5 ) ,高浓度胰岛素组和葡萄糖 +胰岛素组大鼠动脉条合成和分泌ADM明显增加 ,并呈剂量依赖性。结论高浓度胰岛素可刺激大鼠动脉条合成、分泌ADM增加。ObjectiveTo investigate the effect of glucose and insulin on the synthesis and secretion of adrenomedullin (ADM) by rat aortas in vitro.MethodsThe rat aortas were cut into pieces and divided into several equal groups. All the groups were incubated in K-H buffers including different levels of glucose,insulin and insulin+glucose for 3 hours,the group incubated in K-H buffer without glucose was used as control. To determine ADM in K-H buffers and tissues using RIA method.ResultsADM levels in insulin groups (100.0 μIU/ml,200.0 μIU/ml) and insulin+glucose groups were higher than that in control ( P<0.05),the ADM levels in glucose groups and low level insulin group (20.0 μIU/ml) were not significantly difference compared with the control. ConclusionHigh levels of insulin can stimulate the synthesis and secretion of ADM.

关 键 词：葡萄糖 胰岛素 胰岛素抵抗 肾上腺髓质素 

分 类 号：R446.6[医药卫生—诊断学]