加味左归饮对去卵巢骨质疏松大鼠下丘脑雌激素α受体的影响  

The Effect of Zuo Guiyin Modified on the Estrogen Receptor alpha and Its mRNA in the Hypothalamus of Ovariectomized Rats after Osteoporosis

作　　者：郑雨[1] 陈东风[2] 周健洪[2] 黎晖[2] 李伊为[2] 邓汝东[2] 张赛霞[2] 

机构地区：[1]汕头市中心医院药学科,广东汕头515031 [2]广州中医药大学解剖学教研室,广州510405

出　　处：《中药新药与临床药理》2004年第4期229-232,共4页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：基金项目:国家自然科学基金项目(编号30371837);广东省自然科学基金项目(编号31483);广东省中医药管理局项目(编号100086)。

摘　　要：目的观察加味左归饮对绝经后骨质疏松大鼠下丘脑雌激素受体α(ERα)及其基因表达的影响。方法以切除卵巢的雌性大鼠作为骨质疏松的动物模型,将大鼠分为假手术组、模型组、阳性对照组和加味左归饮低、中、高剂量组,用免疫组织化学方法和原位杂交方法检测下丘脑ERα及其mRNA表达。结果模型组ERα受体及其mRNA阳性细胞与假手术组比较显著减少;而加味左归饮能够提高去卵巢后下丘脑ERα及其mRNA阳性细胞数。结论加味左归饮可通过增加去卵巢大鼠下丘脑ERα表达以增强雌激素的生物效应。objective To study the effect of the estrogen receptor α(ERα)and its mRNA in the hypothalamus of ovariectomized rats after osteoporosis.Methods Rats were divided into four groups:shame-operated control group,the ovariectomized group,the positive control group and Chinese herbal group.The change of the ERαand ERαmRNA positive cells in the hypothalamus was studied by immunohistochemistry and in situ hybridization.Results The number of ERαand its mRNA ERαpositive cells in the hypothalamus of the ovariectomized group was less than that of the shame-operated control group;Zuo Guiyin modified increase the number of ERαand its mRNA ERαpositive cells in the hypothalamus of the ovariectomized rats。Conclusion Zuo Guiyin modified increase the expression of ERαin the hypothalamus to enhance estrogen bioactivity。

关 键 词：加味左归饮 去卵巢大鼠骨质疏松 雌激素受体Α 雌激素受体αmRNA 下丘脑 

分 类 号：R285.5[医药卫生—中药学]