光肩星天牛及其近缘种mtDNA序列和基因特点  

A Study on mtDNA Sequence and Its Genetic Characteristics of Anoplophora glabripennis and Its Sibling Species

作　　者：安榆林[1] 黄晓明[2] 杨晓军[1] 林晓佳[3] 嵇保中[3] 王保德[4] Victor C.Mastro 

机构地区：[1]江苏出入境检验检疫局,江苏南京210001 [2]东南大学,江苏南京210009 [3]南京林业大学,江苏南京210037 [4]美国农业部动植物检疫局Otis植物保护实验室

出　　处：《南京林业大学学报（自然科学版）》2004年第4期6-12,共7页Journal of Nanjing Forestry University：Natural Sciences Edition

基　　金：中美合作中国国家质检总局资助项目 (国质检K0 48 2 0 0 0 )

摘　　要：通过对光肩星天牛及其近缘种mtDNA的 7个基因片段的测序 ,分析了光肩星天牛mtDNA的特点。结果表明 ,所测的光肩星天牛mtDNA 7个基因序列与其近缘种差异达 11.3%以上 ,光肩星天牛种群及个体间碱基差异小于 3% ,大部分基因基本没有差异 ,光肩星天牛mtDNA的这一特点说明这些基因在种内较为保守 ,可用于该虫的鉴定和系统遗传分析 ;光肩星天牛与黄斑星天牛在这些基因上差异很小 ,有可能为同一个种。在COⅠ ,Cytb等基因测序中 ,发现部分个体出现差异大的序列 。Seven mitochondrial DNA regions of Anoplophora glabripennis and its sibling species are sequenced to understand the molecular characteristics of mtDNA.The results show that the base variation of the seven mtDNA gene sequences of Anoplophora glabripennis and those of its sibling species is above 11.3%,whereas the base variation among Anoplophora glabripennis species group and its individuals is less than 3%,nearly no variation existed in most genes,these character imply that these mitochondrial genes are conserved intraspeciesly and could be used in identification and phylogenetic analysis of Anoplophora glabripennis.The study also suggest that Anoplophora glabripennis and A.nobilis might belong to same species as their sequence variation in these genes are negligible.In the sequencing of COⅠ,Cytb genes,sequences with significant variation are found in some individuals,sometimes they are similar to those of the sibling.It remains to be elucidated that weather this is due to the presence of nuclear pseudogene,or it may arise from mitochondrial inleakage.

关 键 词：光肩星天牛 近缘种 线粒体DNA 基因 测序 

分 类 号：Q969[生物学—昆虫学]