温胆汤对高脂血症大鼠及小鼠体内脂质代谢调节机理研究  

Mechanism Study of WenDan Decoction on Lipid Metabolism in Hyperlipidemic Adult Mice and Rats

作　　者：李佳楠[1] 陈东辉[1] 罗霞[1] 杨志荣[2] 

机构地区：[1]江汉大学医学与生命科学学院,湖北武汉430056 [2]四川省中药研究所,四川成都610065

出　　处：《江汉大学学报（自然科学版）》2004年第2期62-66,共5页Journal of Jianghan University：Natural Science Edition

摘　　要：目的 :探求温胆汤对实验动物体内脂质代谢调节作用的机理。方法 :采用腹腔注射蛋黄乳剂的方法建立急性高脂血症小鼠模型 ,观察温胆汤对急性高脂血症成年小鼠粪便脂质含量、血清总胆固醇 (TC)、血清总甘油三酯 (TG)、低密度胆固醇 (LDL c)含量、血清丙二醛 (MDA)含量、血清超氧化物歧化酶 (SOD)活性、肝脏总脂解酶(LA)活性、脂蛋白脂肪酶 (LPL)活性和肝脂酶 (HL)活性的影响 ;建立大鼠高脂血症模型 ,观察温胆汤对大鼠血脂(TC、TG)水平、总脂解酶 (LA)、脂蛋白脂酶 (LPL)和肝脂酶 (HL)活性 ,以及肝脏低密度脂蛋白受体 (LDLR)基因表达的影响。结果 :温胆汤可降低小鼠粪便脂质含量 ,显著降低急性高脂血症小鼠血清TC、TG、LDL c含量 (P <0 .0 5 ) ,温胆汤还可提高血清SOD活性 ,降低MDA含量 ,达到降低体内脂质过氧化程度的目的。同时 ,温胆汤可提高肝脏脂蛋白脂肪酶 (LPL)活性和肝脏总脂解酶 (LA) ,但对肝脂酶 (HL)活性无明显影响。在针对高脂血症大鼠的实验研究中发现 ,温胆汤能够显著抑制大鼠血清总胆固醇 (TC)、血清总甘油三酯 (TG)浓度 ,提高脂蛋白脂酶(LPL)和总脂解酶 (LA)活性 ,但对肝脂酶 (HL)活性无明显影响 ;逆转录聚合酶链反应 (RT PCR)实验显示高脂饲料能显著降低大鼠肝脏LDLRmRNA水平 ,温胆汤能显?Objective:In order to explore the mechanism of WenDan Decoction on Lipid Metabolism in experimental mice of acute hyperlipemia model.Methods: To prove the regulating action of WenDan Decoction for serum fat of adult mice, we studied Triglycerides(TG)、Total Cholesterol (TC)、LDL-c、SOD、MDA in serum, and LA、LPL、HL in liver and fat content in faeces of adult mice. On the basis of establishing hyperlipidemia rat model, Triglycerides(TG)、Total Cholesterol (TC) in serum, activities of Lipase (LA)、Lipoprotein Lipase (LPL)、Hepatic Lipase (HL) in liver, parts of hemogram and hepatic LDLR mRNA levels were investigated after 21 days feeding of atherogenic diet.Results: WenDan Decoction can markerly decrease TG、TC、LDL-c、MDA in serum of acute hyperlipermia model mice and increase the action of SOD, LPL and LA,but don't act on HL. The results also suggest that the impact of WenDan Decoction group was better than other groups(P< 0.05). The results suggest that WenDan Decoction significantly reduced the serum TG、TC and increase the activity of LPL and LA, but nullity in HL. The result of RT-PCR test showed that high fat and high cholesterol feeding can significantly induce the reduction of LDLR mRNA levels, while WenDan Decoction can increase hepatic LDLR density.Conclusions:WenDan Decoction can prevent disorder of lipid Metabolism by regulating TC、 TG、 LDL-c through upregaulation of LDLR transcription level and improving ability of antioxidant.

关 键 词：温胆汤 高脂血症 血清总胆固醇 血清总甘油三酯 低密度胆固醇 超氧化物歧化酶 低密度脂蛋白受体 反转录聚合酶链反应 

分 类 号：R541[医药卫生—心血管疾病]