甘-L-脯二肽在仔猪小肠刷状缘膜囊中的抗水解研究  

Hydrolysis of Gly-Pro dipeptide in small intestinal brush border membrane vesicles of piglets

作　　者：代建国[1] 李广京[1] 许柏球[1] 

机构地区：[1]深圳职业技术学院生物应用工程系,广东深圳518055

出　　处：《深圳职业技术学院学报》2004年第2期38-41,共4页Journal of Shenzhen Polytechnic

基　　金：国家自然科学基金资助项目(39870583)

摘　　要：通过孵育试验研究了甘-L-脯(Gly-Pro)二肽在断奶仔猪小肠刷状缘膜囊(Brush Border MemloraneVesides,BBMV)中的水解状况。结果表明,孵育液pH值(分别为3.5,4.0,4.5,5.0,5.5,6.0,6.5,7.0,7.5和8.0)、孵育温度(25℃和37℃)、孵育时间(0.5,1,2,5,10,20,30和40 min)以及BBMV的添加量均不影响此二肽在断奶仔猪小肠BBMV孵育体系中的水解。结果提示,Gly-Pro二肽在断奶仔猪小肠BBMV体系中不水解。In vitro experiments were carried out to examine pH value in incubation solution, incubation temperature and time, and additional amount of BBMV (presumably dipeptidase) on hydrolysis of Gly-Pro dipeptide. The results indicated that pH value in incubation solution (3.5, 4.0, 4.5, 5.0, 5.5, 6.0, 6.5, 7.0, 7.5 and 8.0), incubation temperature (25 ℃ and 37 ℃), incubation time (0.5, 1, 2, 5, 10, 20, 30 and 40 min) and the additional amount of BBMV did not affect hydrolysis of the dipeptide. The results showed that Gly-Pro dipeptide were not hydrolyzed in small intestinal BBMV of weaned piglets.

关 键 词：二肽 断奶仔猪 小肠 刷状缘膜囊 水解 

分 类 号：Q516[生物学—生物化学]