用Dot-ELISA检测马鼻肺炎病毒抗体的研究  

Detection of the Serum Anti-body Against Equine Rhinopneumnoitis Virus by Dot-ELISA

作　　者：晁群芳[1] 

机构地区：[1]新疆大学生命科学与技术学院,新疆乌鲁木齐830046

出　　处：《新疆大学学报（自然科学版）》2004年第3期292-294,共3页Journal of Xinjiang University(Natural Science Edition)

摘　　要：用从德国引进的马鼻肺炎 ( EHV-1 )标准毒株经 BHK-2 1细胞传代增殖 ,免疫家兔 ,制备高免血清 ,提取Ig G并用辣根过氧化物酶 ( HRP)进行标记 ,建立了能够大量、快速、特异地检测马鼻肺炎的斑点 -酶联免疫吸附实验 ( Dot-ELISA)方法 ,并应用此方法对 1 0 0份马血、2份野马流产胎儿病料及从野马流产胎儿病料中分离的毒株 PH93 -1进行了检测 ,结果证明 ,本方法具有较好的特异性和敏感性 ,而且操作简便、快速 ,是一种适用于马鼻肺炎的诊断、流行病学调查。A sensitive and specific diagnostic method for detecting equine rhionpneumonitis virus is developed using dot-ELISA . Equine rhinopneumonitis virus is propagated on BHK-21 cell successfully. The hyperimmune rabbit anti-equine rhinopneumonitis virus IgG raised against equine rhinopneumonitis virus antigen is used as capture anti-body and purified rabbit anti-equine rhinopneumonitis virus IgG labeled with horseradish peroxidase (HRP). The result in the detection of 100 sera of horse shows that they are all negative and sensitive. The virus, (PH93-1) which has been isolated from Prezwalskii horse and two abortive fetuses is examined.

关 键 词：马鼻肺炎病毒 DOT-ELISA 检测 

分 类 号：S852[农业科学—基础兽医学]