春小麦病毒病的鉴定——Ⅱ 生物学特性及血清学鉴定  

IDENTIFICATION OF THE VIRUS DISEASE ON SPRING WHEAT——Ⅱ.Biological Characters and Serological Test

作　　者：刘洪义[1] 吕军[1] 张明厚[1] 

机构地区：[1]东北农学院植物保护系,哈尔滨150030

出　　处：《东北农学院学报》1993年第3期219-223,共5页

基　　金：黑龙江省自然科学基金

摘　　要：试验证明,春小麦病毒病毒原的传毒介体为灰飞虱(Laodelphax striatella Fallen),不能经卵传毒。土壤、种子、蚜虫、叶蝉及汁液磨擦接种均不传毒。生物学测定及田间调查表明,该病毒的症状为矮化、丛生、叶片有条纹花叶、条点及植株早枯。症状表现与感病时期及温度有关,日温高于30℃则隐症。在鉴别寄主上的反应与日本 Toriyama 的报道一致。黍(Panicummiliaceum),无芒雀麦(Bromus inermis Leyss),野稗(Echinochloa crusgalli(L.)Beauv)为新确定的寄主。该病毒在谷子及黍上造成大面积危害,并在谷子上表现特殊症状。ELISA 测定结果表明,该病毒与小麦丛矮病的抗血清呈阳性反应。以上结果及电镜观察(已报道)确定该病毒为北方禾谷花叶病毒(NCMV),又名小麦丛矮病毒(WRSV)。The virus is transmitted by the vector of planthopper(Laodelphax striatella Fallen),butnot transmitted through its eggs.The virus can't be transmitted by seed,aphid,leafhopperand the extract of sap of infected plant.Biological assays and field surveys indicated that thesymptoms of disease were:stunt,rosette,mosaic,streak and early death.The symptom ap-pearance was closely relatcd with the infection period and temperature.The symptom mightbe masking as the temperature was above 30℃.All the infected plants died earlier.The symp-toms on the diagnostic plants were similar to those reperted by Toriyama.Three gramineous plant species were newly proved hosts of the virus in China.They arePanicum miliaceum,Bromus inermis and Echinochloa crusgalli.The virus disease caused severedamage to millet(Setaria italica)and Panicum miliaceum.ELISA tests with the antiserum ofwheat rosetee stunt virus(WESV)were positive.According to the result mentioned above andthe studies on electron microscopy(reported before),the virus was identified as Northern ce-real mosaic virus(NCMV).

关 键 词：小麦 病毒病 灰飞虱 

分 类 号：S435.121[农业科学—农业昆虫与害虫防治]