生长因子(EGF和PDGF-BB)对小鼠骨髓基质干细胞增殖的作用及其意义(英文)  

Effects and significance of EGF and PDGF-BB on proliferation of murine bone marrow stromal

作　　者：阮绪芝[1] 陈霞萍[2] 李瑞明 王为民 周芳 

机构地区：[1]郧阳医学院生物学教研室,湖北十堰442000 [2]郧阳医学院附属太和医院生命科学研究所,湖北十堰442000

出　　处：《中国医学工程》2004年第3期34-36,39,共4页China Medical Engineering

摘　　要：目的探讨表皮生长因子(epidermalgrowthfactor,EGF)和血小板源生长因子(platelet-derivedgrowthfactor,PDGF-BB)对体外培养的小鼠骨髓基质干细胞(murinebonemarrowstromalstemcells,mMSCs)增殖的作用,探索小鼠骨髓基质干细胞体外快速增殖的条件和方法。方法将小鼠骨髓基质干细胞进行分离和纯化培养后,加入EGF和PDGF-BB,利用3H胸腺嘧啶核苷(3H-TdR)掺入反映细胞增殖效果,倒置显微镜下观察细胞增殖及分化状态。结果EGF和PDGF-BB干预后,3H-TdR掺入(11456.14±968.86)dpm较对照组(3271.88±420.91)dpm明显增高,说明EGF和PDGF-BB可促进小鼠骨髓基质干细胞的增殖;倒置显微镜下观察可见EGF和PDGF-BB处理组mMSCs具有增殖优势,分化相对延迟,而对照组提前显示出自然分化现象。结论生长因子(EGF和PDGF-BB)可促进小鼠骨髓基质干细胞的增殖,是小鼠骨髓基质干细胞体外大量培养和快速增殖的有效刺激因子。Objective: To investigate the effects of EGF, PDGF-BB on the proliferation in cultured murine bone marrow stromal stem cells(mMSCs) and to find a well-proliferation condition and method of murine bone marrow stromal stem cells in vitro. Methods: Murine bone marrow stromal stem cells were isolated and cultured in vitro before EGF and PDGF-BB were added in. Proliferation was measured with 3H-Thymidine incorporation. Results: EGF(10 ng/ml) and PDGF-BB(10 ng/ml) cause increases of 3H-TdR incorporation (11456.14±968.86)dpm more than the control group (3271.88±420.91)dpm significatively(P<0.05). Conclusions: EGF and PDGF-BB were effective and available for mMSCs proliferation in vitro.

关 键 词：小鼠 骨髓基质干细胞 细胞培养 EGF PDGF-BB 表皮生长因子 血小板源生长因子 mMSCs 细胞增殖 

分 类 号：R457.7[医药卫生—治疗学]