白细胞介素-1受体拮抗剂与气道高反应性关系的研究  

Research on relationship between interleukin-1 receptor antagonist and airway hyperresponsiveness

作　　者：陈雪芹[1] 曹霞[1] 杨炯[1] 曹作炎[1] 

机构地区：[1]武汉大学人民医院呼吸内科

出　　处：《中国临床药理学与治疗学》2004年第7期759-762,共4页Chinese Journal of Clinical Pharmacology and Therapeutics

摘　　要：目的 :探讨白细胞介素 1受体拮抗剂 (IL 1ra)与哮喘的气道高反应性 (AHR)的关系。方法 :正常豚鼠 ,先腹腔注射卵清蛋白 (OA)致敏而后雾化吸入OA激发豚鼠哮喘 (哮喘组 ) ,激发前 30min静脉注射IL 1ra 1mg·kg-1(哮喘 +IL 1ra组 ) ,测定豚鼠的气道反应性 (组胺的PC2 0 值 ) ,并收集支气管肺泡灌洗液 (BALF)进行细胞总数计数及分类记数。结果 :哮喘 +IL 1ra组PC2 0 值与对照组无显著性差异 (P >0 .0 5 ) ,显著高于哮喘组 (P <0 .0 1) ;BALF中的中性粒细胞 (NES)的百分比显著低于哮喘组 (P <0 .0 1)。所有豚鼠均存在 IgPC2 0 值与BALF中的NES的百分比呈正相关 (r =0 .95 ,P <0 .0 0 1)。结论 :IL 1ra能降低AHR ,其机制可能与ILAIM: To explore the relationship between interleukin-1receptor antagonist (IL-1ra) and airway hyperresponsiveness (AHR) of asthma. METHODS: The airway responsiveness to inhaled histamine as PC 20 was evaluated among the normal guinea pigs, and sensitized with ovalbumine (OA) and challenged 14 days later (athma group), and injected IL-lra 1 mg·kg -1 intravenously 30 minutes before challenged (asthma plus IL-lra group). The numbers of different cells was counted and classified in bronchoalveolar lavage fluid (BALF). RESULTS: The PC 20 of the asthma plus IL-1ra group was higher than that of asthma group (P< 0.01), and the percentage of the neutrophils (NES) in BALF of the asthma plus IL-1ra group was lower than that of the asthma group (P< 0.01). There was a significant correlation between -IgPC 20 and the percentage of the NES in BALF in all animals (r= 0.68, P< 0.001). CONCLSION: IL-1ra can attenuate airway hyperresponsiveness, possibly by decreasing the airway infiltration of neutrophils.

关 键 词：白细胞介素-1受体拮抗剂 哮喘 气道高反应性 中性粒细胞 

分 类 号：R965.1[医药卫生—药理学] R976[医药卫生—药学]