水飞蓟宾对抗大鼠酒精性脂肪肝作用及机制  

Effects ant mechanism of silibinin on antagonizing alcoholic fatty liver in rats

作　　者：谭海荣 吴谦[2] 潘竞锵 肖柳英 韩超 林洁茹[3] 郑琳颖[3] 李博萍[3] 

机构地区：[1]广州市中医中药研究所 [2]广东省药物研究所 [3]广州市中医医院

出　　处：《中国临床药理学与治疗学》2004年第7期803-806,共4页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：广东省中医药局滚动资助项目 (№ 3 0 2 0 0 3 ) ;广州市中医药;中西医结合课题资助项目 (№ 2 0 0 2 3 7)

摘　　要：目的 :观察水飞蓟宾 (Silbinin)对大鼠酒精性脂肪肝作用及其机制。方法 :饮用 10 %乙醇 ,加高脂高热量饲料喂饲 6周 ,造成大鼠酒精性脂肪肝模型 ;灌胃给药水飞蓟宾 10 0mg·kg-1,继续酒精和高脂高热量饲料处理 4周后 ,处死大鼠 ,测定血清天冬氨酸氨基转移酶 (AST)、丙氨酸氨基转移酶 (ALT)、碱性磷酸酶 (AKP)活性和甘油三酯 (TG)、胆固醇(TC)、低密度脂蛋白 胆固醇 (LDL C)、高密度脂蛋白 胆固醇 (HDL C)、大鼠肿瘤坏死因子 α(TNF α)、大鼠转化生长因子 β1(TGF β1)含量 ,测定肝细胞TG、肝匀浆丙二醛 (MDA)、超氧化歧化酶 (SOD)、还原性谷胱甘肽 (GSH)水平以及肝组织学检查。结果 :水飞蓟宾能抑制病鼠血清AST、ALT、AKP活性 (P <0 .0 5或 0 .0 1)和降低TG、LDL C、TNF α、TGF β1含量(P <0 .0 5或 0 .0 1) ,提高HDL C含量 ;降低肝匀浆TG和MDA含量 (P <0 .0 5或 0 .0 1) ,增强SOD活性(P <0 .0 1)以及改善肝细胞水变性和脂肪变性 (P <0 .0 1)。结论 :水飞蓟素阻止大鼠酒精性脂肪肝形成 ,其作用机制包括 :抗氧化、清除自由基代谢产物 ,抑制脂质过氧化反应 ;调血脂、减少脂肪在肝脏的沉积 ;AIM: To investigate the effects and mechanism of silibinin(Sil)on rats with alcoholic fatty liver. METHODS: The animal models with alcoholic fatty liver were prepared in rats by feeding on high caloric food and drinking 10% alcohol qd for 10 weeks; after 6-week treatment, the effects of Sil (intragastrointestinally treated 100 mg·kg -1, qd for 4 weeks) were observed by serum activities of alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (AKP), and serum contents of total cholesterols (TC) , triacylglycerol (TG), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), tumor necrosis factor (TNF)-α and transforming growth factor (TGF)-β 1, and the contents of TG, malondialdehyde (MDA), glutathione (GSH), and activity of superoxide dismutase (SOD) in the hepatic tissue. Hepatic histopathology was observed by microscope. RESULTS: Serum levels of ALT, AST and AKP decreased in administration of Sil (P< 0.05 or 0.01), the levels of TG, LDL-C, TNF-α and TGF-β 1 reduced (P< 0.05 or 0.01), the concentrations of TG and MDA in the hepatic tissue lowered (P< 0.01), and the activity of SOD potentiated (P< 0.01). Hydropic and/or fatty degeneration of hepatocytes were improved (P< 0.01). CONCLUSION: Silibinin can preclude alcoholic fatty liver in rats from drinking alcohol and feeding high caloric food, and pharmacological mechanisms were involved in anti-oxidation and removing free radical, inhibiting lipid peroxidation, adapting blood lipid component, reducing fatty sediment in liver, anti-inflammation and anti-hyperplasia.

关 键 词：水飞蓟宾 酒精性脂肪肝 自由基损伤 肿瘤坏死因子-Α 转化生长因子-Β1 

分 类 号：R965.1[医药卫生—药理学] R975.5[医药卫生—药学]